您好，欢迎访问广西农业科学院 机构知识库！

Non-covalent interaction of complex plant protein and betanin: Mechanism of improving thermal stability of betanin

文献类型： 外文期刊

作者： Hu, Ting 1 ; Dai, Taotao 1 ; He, Xuemei 2 ; Deng, Lizhen 1 ; Li, Ti 1 ; Sun, Jian 2 ; Liu, Chengmei 1 ; Chen, Jun 1 ;

作者机构： 1.Nanchang Univ, State Key Lab Food Sci & Technolog, 235 Nanjing East Rd, Nanchang 330047, Peoples R China

2.Guangxi Acad Agr Sci, Nanning 530007, Peoples R China

关键词： Betanin; Thermal stability; Complex plant protein; Interaction

期刊名称：FOOD HYDROCOLLOIDS （ 影响因子：10.7； 五年影响因子：10.9 ）

ISSN： 0268-005X

年卷期： 2023 年 138 卷

页码：

收录情况： SCI

摘要： Betanin (BN) is a promising purple-red natural pigment, but its application is hindered by thermal instability. The complex plant protein (RP-SPI) constructed by rice protein (RP) and soy protein isolate (SPI) was used as raw material to investigate the thermal protection effect and protective mechanism of RP-SPI on BN. The results showed that RP-SPI significantly improved the thermal stability of BN, and the retention rate of BN increased from 5.63% to 48.08% when heated at 80 degrees C for 60 min. Subsequently, the fluorescence spectroscopy, isothermal titration calorimetry (ITC), and molecular docking were utilized to investigate the interaction mechanism between RP-SPI and BN. The results indicated that the main interaction between RP-SPI and BN was hydrogen bond. The interaction changed the secondary structure and reduced surface hydrophobicity of the protein. Meanwhile, morphological observation showed that RP-SPI was a flake protein with a network structure, and a large amount of aggregation occurred after binding with BN, resulting in a significant increase in particle size. Taking together, the non-covalent interaction is the key to the thermal protection of RP-SPI on BN. Betanin bound to RP-SPI through hydrogen bonds and acted as a bridge connecting the surrounding proteins.

  • 相关文献

[1]Complex plant protein prepared from rice protein and pea protein: Improve the thermal stability of betanin. Hu, Ting,Chen, Jun,Liu, Chengmei,Dai, Taotao,He, Xuemei,Tang, Yayuan,Sun, Jian,Dai, Taotao. 2023

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广西农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台