Dual DNAzyme-MoS2/GDY Catalytic Assembly Enables Smartphone-Based Multiplex Detection of Sugarcane Pokkah Boeng Pathogens at Sub-Femtomolar Levels
文献类型： 外文期刊
作者： Fu, Bingtao 1 ; Che, Rongshuai 1 ; Wang, Zeping 2 ; Feng, Defen 1 ; Yan, Jun 1 ; Huang, Ke-Jing 1 ; Ya, Yu 2 ; Tan, Xuecai 1 ;
作者机构： 1.Guangxi Minzu Univ, Engn Res Ctr Low Carbon & High Qual Utilizat Fores, Educ Dept Guangxi Zhuang Autonomous Reg, Sch Chem & Chem Engn,State Ethn Affairs Commiss, Nanning 530006, Peoples R China
2.Guangxi Acad Agr Sci, Nanning 530007, Peoples R China
期刊名称：ANALYTICAL CHEMISTRY （ 影响因子：6.7； 五年影响因子：6.6 ）
ISSN： 0003-2700
年卷期： 2025 年 97 卷 34 期
页码：
收录情况： SCI
摘要： Rapid on-site detection of sugarcane pokkah boeng disease caused by Fusarium pathogens remains challenging due to the lack of portable platforms combining high sensitivity and multiplexing capability. Here, we present a self-powered biosensor integrating a dual DNAzyme-driven catalytic system with a MoS2/graphdiyne (GDY) nanohybrid-modified biofuel cell (EBFC) for simultaneous detection of Fusarium sacchari and Fusarium verticillioides. The key innovation lies in the windmill-shaped dual DNAzyme structure that enables Mn2+/Mg2+-dependent target recycling, synergistically coupled with the hybridization chain reaction (HCR) and triplex catalytic hairpin assembly (TCHA) for exponential signal amplification. The MoS2/GDY nanohybrid provides an ideal conductive substrate with 3.8-fold higher DNA loading capacity than pristine MoS2, while the integration of a charge-storage capacitor boosts detection sensitivity by 10.4- and 9.8-fold compared with conventional EBFCs through transient current amplification. The smartphone-coupled system achieves unprecedented detection limits of 21.3 aM (F. sacchari) and 54.3 aM (F. verticillioides) with a dynamic range spanning 5 orders of magnitude (0.1 fM-10 nM), demonstrating excellent specificity against non-target pathogens (more than 95% signal discrimination). This smartphone-integrated biosensor represents a field-ready diagnostic tool for rapid on-site screening of sugarcane fungal pathogens, offering a transformative approach to mitigate crop losses through early disease intervention and precision agriculture management.
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