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Overexpression of sugarcane SoTUA gene enhances cold tolerance in transgenic sugarcane

文献类型： 外文期刊

作者： Chen, Jiao-Yun 1 ; Khan, Qaisar 1 ; Sun, Bo 1 ; Tang, Li-Hua 1 ; Yang, Li-Tao 1 ; Zhang, Bao-Qing 2 ; Xiu, Xing-Yong 1 ;

作者机构： 1.Guangxi Univ, Agr Coll, Nanning 530004, Peoples R China

2.Chinese Acad Agr Sci, Guangxi Acad Agr Sci,Key Lab Sugarcane Biotechnol, Sugarcane Res Inst,Sugarcane Res Ctr, Minist Agr,Guangxi Key Lab Sugarcane Genet Improv, Nanning 530007, Peoples R China

3.South China Agr Univ, Agr Coll, Guangzhou 510640, Peoples R China

期刊名称：AGRONOMY JOURNAL （ 影响因子：2.24； 五年影响因子：2.829 ）

ISSN： 0002-1962

年卷期：

页码：

收录情况： SCI

摘要： Alpha (alpha)-tubulin (TUA) is a primary unit of cytoskeleton microtubules. In recent years, more frequent frosts have caused considerable yield loss in sugarcane (Saccharum spp. hybrid) production. Temperature is one of the most influential abiotic factors affecting the microtubule polymerization state in plants. In this research, we developed overexpressing SoTUA transgenic sugarcane lines from the cold-susceptible cultivar ROC22. The transgene was confirmed by polymerase chain reaction (PCR) and quantificational real-time polymerase chain reaction (qRT-PCR) analysis, while the relative expression of SoTUA protein was verified by Western blot. Compared with the nontransformed control plants (wild type [WT]), the transgenic lines showed higher relative protein expression than the WT at 25 degrees C, which indicates the overexpression of SoTUA protein. The contents of soluble protein and sugar and the activity of peroxidase (POD) were higher while the content of maleicdialdehyde was lower in the transgenic plants than the WT plants under the chilling treatment. Meanwhile, the expression levels of cold-defense related genes (P5CS, WRKY, Cu/Zn-SOD, and POD1) were higher in the transgenic lines than in the WT. These results suggest that the SoTUA protein plays an important role in protecting plants during chilling stress. This positive effect is not affected by transgenic plant generations.

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