Bimodal 3D DNA nanomachines coupling with bioconjugates powered electrochemical and visual-driven dual-enzyme cascade sensing of Let-7a
文献类型： 外文期刊
作者： Tang, Danyao 1 ; Shi, Jinyue 1 ; Huang, Yumei 1 ; Wu, Yeyu 1 ; Wu, Jiawen 1 ; Luo, Hu 1 ; Huang, Ke-Jing 1 ; Tan, Xuecai 1 ; Ya, Yu 2 ;
作者机构： 1.Guangxi Minzu Univ, Guangxi Key Lab Chem & Engn Forest Prod,Guangxi Co, Sch Chem & Chem Engn,Key Lab Chem & Engn Forest Pr, Lab Opt Elect Chemo Biosensing & Mol Recognit,Educ, Nanning 530006, Peoples R China
2.Guangxi Acad Agr Sci, Inst Agr Prod Qual Safety & Testing Technol, Nanning 530007, Peoples R China
关键词： Bimodal 3D DNA nanomachine; AuNPs/carbon/molybdenum disulfide; bioconjugates; Graphene/carbon cloth flexible electrode; Tumor biomarkers
期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：8.4； 五年影响因子：7.2 ）
ISSN：
年卷期： 2024 年 413 卷
页码：
收录情况： SCI
摘要： A dual-enzyme self-powered biosensor utilizing bimodal 3D DNA nanomachines and gold nanoparticle (AuNPs)/ carbon/disulfide molybdenum hollow nanorods bioconjugates is developed for the electrochemical and visual detection of the tumor biomarker let-7a. DNA probes are immobilized on a flexible electrode substrate of AuNPs/ graphdiyne/carbon cloth to improve probe loading capacity and electron transfer efficiency. The presence of let7a initiates the work of dual-enzyme 3D DNA nanomachine, and the output DNA single strand cleaved by Nb. BbvCI is served as a bridge to connect glucose oxidase (GOD)-DNA conjugate. The GOD coordinates with the cathodic enzyme bilirubin oxidase and produces an increased open circuit voltage signal, which is attributed to the hexaammine ruthenium in the double-stranded adsorption system of the biocathode. By utilizing the H2O2 generated during the oxidation process of GOD, a linear relationship between the color of 3,3 ',5,5 '-tetramethylbenzidine and the concentration of let-7a is established, enhancing the reliability of the detection results through the independent signal conversion mechanisms of electrochemical/colorimetric modes. The results demonstrate a wide dynamic range of 0.0001-0.1 pM with detection limits of 31.04 aM (S/N=3) in electrochemical mode and 59.99 aM (S/N=3) in the colorimetric mode. This dual-mode biosensing strategy can be expanded to detect additional nucleic acid biomarkers, providing a promising support and reference for biological analysis and medical diagnosis.
- 相关文献
作者其他论文 更多>>
-
A dual-mode strategy for early detection of sugarcane pokkah boeng disease pathogen: A portable sensing device based on Cross-N DNA framework and MoS2@GDY
作者：Song, Yujie;Wu, Qingnian;Su, Jing;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Wang, Zeping;Liao, Jie;Zhang, Xiaoqiu;Ya, Yu
关键词：Portable dual-mode detection device; Exo III exonuclease-assisted CHA strategy; Cross-N DNA framework; Sugarcane pokkah boeng disease pathogen; Smartphone-assisted analysis
-
An intelligent sensing platform for sugarcane pokkah boeng disease based on CHA-driven RCA cascade DNAzyme amplification technology and clustered DNA nanoflowers
作者：Wu, Qingnian;Song, Yujie;Jin, Chenchen;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Ya, Yu;Wang, Zeping;Liao, Jie;Yan, Feiyan
关键词：Sugarcane pokkah boeng disease; Cascade amplification technology; Bundle DNA nanoflowers; Smartphone assisted detection
-
Sensitivity-enhanced self-powered biosensing platform for detection of sugarcane smut using Mn-doped ZIF-67, RCA-DNA nano-grid array and capacitor
作者：Fu, Bingtao;Che, Rongshuai;Wu, Yeyu;Luo, Hu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Yan, Feiyan;Ya, Yu
关键词：Sugarcane smut; Self-powered platform; DNA nano-grid array; Cascade signal amplification
-
From leaf to lab-on-cloth: Spatial DNA nanorobotics and 2D graphyne synergy enable ultra-precise electrochemical tracking of sugarcane pokkah boeng disease
作者：Wu, Qingnian;Jin, Chenchen;Zhao, Yinxia;Feng, Defen;Huang, Ke-Jing;Tan, Xuecai;Ya, Yu;Yan, Feiyan;Xie, Shengyu
关键词：Sugarcane pokkah boeng disease; S-GDY; Dual strand displacement amplification; Dual-signal readout; Spatial DNA nanorobotics
-
High-Performance Biosensing Platforms Based on Enzyme-Linked Nucleic Acid Amplification Regulated by Synergistic Allosteric Hairpin Catalysis of Bimetallic Nanozymes and Its Mechanisms
作者：Ma, Yunzhi;Jin, Chenchen;Wu, Yeyu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Yan, Feiyan;Ya, Yu
关键词：
-
Super signal-enhancement biosensing platform for precise target recognition based on rolling circle-hybridization chain dual linear cascade amplification technology
作者：Tang, Danyao;Xiong, Chunyuan;Wu, Yeyu;Luo, Hu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Ya, Yu
关键词：Double-layer linear cascade amplification technology; Precise target identification; Super signal amplification; Efficient conversion
-
Highly Sensitive Detection of Water in Organic Solvents Using Pyrene-Phenol-based Fluorescent Probe
作者：Liang, Jing;Wang, Yan-Li;Jiang, Cui-Wen;Huang, Xiao-Chuan;Tang, Li;Li, Tao;Ya, Yu;Ning, De-Jiao;Xie, Li-Ping;Yan, Fei-Yan
关键词：Fluorescent probe; Pyrene-phenol; Differentiation of solvents; Detection of water content
