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Exon based amplified polymorphism (EBAP): A novel and universal molecular marker for plants

文献类型： 外文期刊

作者： Xiong, Faqian 1 ; Liu, Jing 2 ; Tang, Ronghua 2 ; Yang, Taiyi 2 ; Yang, Xinghai 4 ; He, Liangqiong 2 ; Han, Zhuqiang 2 ; Qiu, Lihang 3 ; Zou, Chenglin 2 ; Tang, Xiumei 2 ; Luo, Cong 1 ; Zhong, Ruichun 2 ; Jiang, Jing 2 ; Huang, Zhipeng 2 ; Wu, Haining 2 ; Liu, Junxian 3 ; He, Xinhua 1 ;

作者机构： 1.Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Peoples R China

2.Guangxi Acad Agr Sci, Cash Crops Res Inst, Nanning 530007, Peoples R China

3.Guangxi Acad Agr Sci, Key Lab Sugarcane Biotechnol & Genet Improvement, Guangxi Key Lab Sugarcane Genet Improvement, Sugarcane Res Inst,Minist Agr & Rural Affairs, Nanning 530007, Peoples R China

4.Guangxi Acad Agr Sci, Rice Res Inst, Nanning 530007, Peoples R China

关键词： Exon based amplified polymorphism (EBAP); Molecular marker; Exon; Fingerprinting

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.826； 五年影响因子：3.511 ）

ISSN： 0717-3458

年卷期： 2022 年 56 卷

页码：

收录情况： SCI

摘要： Background: Exons are an important part of genes. However, there has been no molecular marker technique based on single primer amplification developed for exons region. Results: A novel molecular marker technique called exon based amplified polymorphism (EBAP) which used single primer to perform the PCR was developed. Single primers were designed based on the rich GC bases in the exon region of genes. The single primer consists of filling sequence, intermediate core region sequence and selective bases sequence. The filling sequence has a total of six bases, which can be 2A and 2T or 3A and 3T. The intermediate core region sequence consists of eight bases, all of which are G and C, but at least 2G or 2C are required. The first of the three selective bases must be either A or T, and the second and third are arbitrary bases. Due to the principle of primer design, these single primers should be universal. We applied it to the DNA polymorphisms detection of maize, sugarcane, potato, cassava, cabbage, and peanut. The results showed that it detected more abundant DNA polymorphisms in the first five crops, but not in the cultivated peanut. In addition, different band patterns were obtained by amplifying peanut cDNA with different single primers. Conclusions: We have developed a novel and universal molecular marker technique. It is simple, fast and efficient. It does not require genomic sequence information. It can be widely used in germplasm identification, genetic diversity analysis, and molecular fingerprinting. (c) 2022 Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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