A comparative investigation of the interaction and pharmacokinetics of hemoglobin with berberine and its oxymetabolite
文献类型： 外文期刊
作者： Chen, Han-Bin 1 ; Luo, Chao-Dan 4 ; Ai, Gao-Xiang 5 ; Wang, Yong-Fu 10 ; Li, Cai-Lan 11 ; Tan, Li-Hua 5 ; Lee, Simon Min 1 ;
作者机构： 1.Guangzhou Univ Chinese Med, Affiliated Hosp Chinese Med 1, Guangzhou 510405, Peoples R China
2.Univ Macau, Inst Chinese Med Sci, State Key Lab Qual Res Chinese Med, Macau, Peoples R China
3.Hong Kong Polytech Univ, Dept Rehabil Sci, Muscle Physiol Lab, Hong Kong, Peoples R China
4.Guangxi Acad Agr Sci, Guangxi Inst Subtrop Agr Prod Proc, Guangxi Subtrop Crops Res Inst, Nanning 530001, Peoples R China
5.Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou 510006, Peoples R China
6.Guangdong Univ Technol, Sch Biomed & Pharmaceut Sci, Guangzhou 510006, Peoples R China
7.Guangzhou Univ Chinese Med, Affiliated Hosp 2, Guangzhou 510120, Peoples R China
8.Guangzhou Univ Chinese Med, State Key Lab Dampness Syndrome Chinese Med, Affiliated Hosp 2, Guangzhou 510120, Peoples R China
9.Guangdong Prov Key Lab Clin Res Tradit Chinese Me, Guangzhou 510120, Peoples R China
10.Guangzhou Univ Chinese Med, Sch Clin Med 1, Guangzhou 510120, Peoples R China
11.Zunyi Med Univ, Dept Pharmacol, Zhuhai Campus, Zhuhai 519041, Peoples R China
关键词： Berberine; Interaction; Metabolite; Pharmacokinetics; Hemoglobin
期刊名称：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS （ 影响因子：3.209； 五年影响因子：3.132 ）
ISSN： 0731-7085
年卷期： 2021 年 199 卷
页码：
收录情况： SCI
摘要： Berberine (BBR), isolated from Coptis chinensis, is one type of isoquinoline alkaloids. BBR exerts numerous of bioactivities but the plasma concentration is really low. In our previous study, a new oxymetabolite (OBB) has been discovered and showed superior anti-inflammatory effect comparing with BBR. The aim of this study is to investigate the interaction, metabolite and pharmacokinetics of BBR with hemoglobin. Sprague-Dawley rats were used to carry out the interaction, metabolite and pharmacokinetics of BBR and OBB in vivo. Fluorescence spectra were used to analyse the interaction in vitro. Results showed that OBB could be generated after intravenous injection or incubating with BBR in vitro and in vivo; Both BBR and OBB exerted much stronger binding interaction with hemoglobin than plasma and affect the conformation of bovine hemoglobin and change the fluorescence spectral properties; BBR and OBB were mainly presented and transported in the proteins-bound form. These results provide a new insight to understand the dynamic equilibrium of BBR and OBB within body from the perspective of new metabolic pathways. (c) 2021 Elsevier B.V. All rights reserved.
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