您好,欢迎访问广西农业科学院 机构知识库!

Microsatellite Enrichment from AFLP Fragments by Magnetic Beads

文献类型: 外文期刊

作者: Gao, GQ 1 ; He, GH 1 ; Li, YR 1 ;

作者机构: 1.Guangxi Acad Agr Sci, Lab Crop Mol Genet Improvement, Nanning 530007, Peoples R China; Tuskegee Univ, Biotechnol Res Ctr, Tuskegee, AL 36083 USA

关键词: microsatellite;AFLP;magnetic beads;peanut

期刊名称:ACTA BOTANICA SINICA ( 影响因子:0.599; )

ISSN:

年卷期:

页码:

收录情况: SCI

摘要: A simple and efficient method was presented for isolating microsatellite DNA markers from peanut (Arachis hypogaea L) genome. The genomic DNA was converted into pre-amplified AFLP fragments and hybridized with biotin-labeled SSR probes. Then the hybrid mixture was used to incubate with magnetic beads coated with streptavidin. After washing to remove the non-SSR fragments, the eluted single-strand DNA, which was cloned and sequenced, was largely enriched for microsatellites. Primers can then be designed according to the sequence flanking the repeat motifs and used for polymorphism analysis. The whole experiment can be completed within one week and can be employed as a reliable option for any molecular laboratory to develop SSR markers.

  • 相关文献

[1]Development of Pathogenicity and AFLP to Characterize Fusarium oxysporum f.sp momordicae Isolates from Bitter Gourd in China. Li, Qi-Qin,Yuan, Gao-Qing,Chen, Zhen-Dong,Huang, Ru-Kui,Wen, Jun-Li,Chen, Zhen-Dong,Huang, Ru-Kui.

[2]Nitric oxide suppresses aluminum-induced programmed cell death in peanut (Arachis hypoganea L.) root tips by improving mitochondrial physiological properties. Huang, Wenjing,Oo, Thet Lwin,Gu, Minghua,Zhan, Jie,Wang, Aiqin,He, Long-Fei,He, Huyi,He, Long-Fei. 2018

[3]Intercropping in Sugarcane Cultivation Influenced the Soil Properties and Enhanced the Diversity of Vital Diazotrophic Bacteria. Wang, Fei-Yong,Li, Chang-Ning,Lan, Tao-Ju,Li, Yang-Rui,Wang, Fei-Yong,Li, Chang-Ning,Li, Yang-Rui,Wang, Zhen,Wang, Fei-Yong,Singh, Rajesh Kumar,Singh, Pratiksha,Yang, Li-Tao. 2017

[4]Simple sequence repeat markers for botanical varieties of cultivated peanut (Arachis hypogaea L.). Meng, RH,Gao, H,Guo, BZ,Gao, GQ,Newman, M,Pittman, RN,Prakash, CS. 2005

[5]Molecular Characterization of High Plant Species Using PCR with Primers Designed from Consensus Branch Point Signal Sequences. Xiong, Faqian,Jiang, Jing,Han, Zhuqiang,Zhong, Ruichun,He, Liangqiong,Tang, Ronghua,Zhuang, Weijian. 2011

[6]Rapid gene expression change in a novel synthesized allopolyploid population of cultivated peanutxArachis doigoi cross by cDNA-SCoT and HFO-TAG technique. Tang Rong-hua,Jiang Jing,Xiong Fa-qian,Huang Zhi-peng,Wu Hai-ning,Gao Zhong-kui,Zhong Rui-chun,Han Zhu-qiang,He Xin-hua. 2017

[7]SSR marker development from peanut gynophore transcriptome sequencing. Tang, Ronghua,Zhou, Meili,Zhao, Chuanzhi,Hou, Lei,Li, Changsheng,Wang, Xingjun,Xia, Han,Wang, Xingjun.

[8]Allergen composition analysis and allergenicity assessment of Chinese peanut cultivars. Zhou, Ningling,Li, Xin,Yang, Anshu,Tong, Ping,Chen, Hongbing,Wu, Zhihua,Zhou, Ningling,Yang, Anshu,Chen, Hongbing,Zhou, Ningling,Li, Xin,Xiong, Faqian,Tang, Ronghua.

[9]Mitochondrial alterations during Al-induced PCD in peanut root tips. Li, Wen,He, Hu-yi,Li, Chuang-zhen,He, Long-fei,He, Hu-yi.

[10]Purification and Recombinant Expression of Major Peanut Allergen Ara h 1. Yan, Fei,Wei, Xiaonan,Li, Xin,Tong, Ping,Chen, Hongbing,Wu, Zhihua,Yang, Anshu,Chen, Hongbing,Yan, Fei,Tang, Ronghua.

[11]Aluminum-induced programmed cell death promoted by AhSAG, a senescence-associated gene in Arachis hypoganea L.. He, Hu-Yi,Wang, Tian-Ju,Wang, Ai-Qin,Li, Chuang-Zhen,He, Long-Fei,He, Hu-Yi.

[12]Nitric oxide inhibits aluminum-induced programmed cell death in peanut (Arachis hypoganea L.) root tips. Huang, Wenjing,Oo, Thet Lwin,Gu, Minghua,He, Long-Fei,He, Huyi,He, Long-Fei.

[13]甘蔗(Saccharum officinarum L.)品种遗传差异的AFLP分子标记分析. 谭裕模,李杨瑞,李容柏,高国庆. 2004

[14]甘蔗种质资源AFLP研究的引物筛选. 李鸣,梁俊,方位宽,李杨瑞,何为中,谭裕模. 2006

[15]用AFLP分子标记技术鉴别甘蔗(Saccharum of ficinarum L.)品种. 谭裕模,李杨瑞,李容柏,高国庆. 2003

[16]不同种质荔枝基因组DNA提取研究. 彭宏祥,廖惠红. 2009

[17]斑茅割手密复合体杂交利用过程野生特异基因遗传分析. 高轶静,张保青,黄玉新,段维兴,杨翠芳,王泽平,张革民. 2019

[18]苦瓜AFLP和SRAP的PCR反应体系优化及应用比较. 黄如葵,黄玉辉,刘萌芽,冯诚诚. 2013

[19]用磁珠富集法从AFLP片段中分离微卫星DNA标记(英文). HE Guo-Hao,李杨瑞. 2003

[20]用AFLP标记分析广西龙眼种质遗传多样性. 李冬波,朱建华,李江舟,曹辉庆,潘丽梅. 2008

作者其他论文 更多>>