您好，欢迎访问广西农业科学院 机构知识库！

Transcriptome Analysis of Sugarcane Young Leaves and Protoplasts after Enzymatic Digestion

文献类型： 外文期刊

作者： Zhang, Demei 1 ; Wang, Rui 1 ; Han, Shijian 1 ; Li, Zhigang 1 ; Xiao, Jiming 1 ; Li, Yangrui 4 ; Wang, Lingqiang 1 ; Li, Suli 1 ;

作者机构： 1.Guangxi Univ, Coll Agr, Guangxi Key Lab Sugarcane Biol, 100 Daxue Rd, Nanning 530004, Peoples R China

2.Guangxi Univ, Guangxi Coll, 100 Daxue Rd, Nanning 530004, Peoples R China

3.Guangxi Univ, Univ Key Lab Crop Cultivat & Tillage, 100 Daxue Rd, Nanning 530004, Peoples R China

4.Guangxi Acad Agr Sci, 174 Daxue Rd, Nanning 530007, Peoples R China

关键词： sugarcane; protoplast; transcriptome; enzymatic digestion

期刊名称：LIFE-BASEL （ 影响因子：3.251； ）

ISSN：

年卷期： 2022 年 12 卷 8 期

页码：

收录情况： SCI

摘要： Sugarcane somatic cell hybridization can break through the barrier of genetic incompatibility between distantly related species in traditional breeding. However, the molecular mechanisms of sugarcane protoplast regeneration and the conditions for protoplast preparation remain largely unknown. In this study, young sugarcane (ROC22) leaves were enzymatically digested, and the viability of protoplasts reached more than 90% after enzymatic digestion (Enzymatic combination: 2% cellulase + 0.5% pectinase + 0.1% dissociative enzyme + 0.3% hemicellulase, pH = 5.8). Transcriptome sequencing was performed on young sugarcane leaves and protoplasts after enzymatic digestion to analyze the differences in gene expression in somatic cells before and after enzymatic digestion. A total of 117,411 unigenes and 43,460 differentially expressed genes were obtained, of which 21,123 were up-regulated and 22,337 down-regulated. The GO terms for the 43,460 differentially expressed genes (DEGs) were classified into three main categories: biological process, cellular component and molecular function, which related to developmental process, growth, cell proliferation, transcription regulator activity, signal transducer activity, antioxidant activity, oxidative stress, kinase activity, cell cycle, cell differentiation, plant hormone signal transduction, and so on. After enzymatic digestion of young sugarcane leaves, the expressions of GAUT, CESA, PSK, CyclinB, CyclinA, CyclinD3 and cdc2 genes associated with plant regeneration were significantly down-regulated to 65%, 47%, 2%, 18.60%, 21.32%, 52% and 45% of young leaves, respectively. After enzymatic digestion, Aux/IAA expression was up-regulated compared with young leaves, and Aux/IAA expression was 3.53 times higher than that of young leaves. Compared with young leaves, these key genes were significantly changed after enzymatic digestion. These results indicate that the process of somatic enzymatic digestion process may affect the regeneration of heterozygous cells to a certain extent.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广西农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台