High-density genetic map and quantitative trait loci map of fruit-related traits in wax gourd (Benincasa hispida)
文献类型： 外文期刊
作者： Su, Liwen 1 ; Gou, Jiquan 1 ; Lv, Haixuan 1 ; Cheng, Zhikui 1 ; Ma, Lianlian 1 ; Huang, Xiaochun 1 ; Wu, Wenting 1 ; Yu, Wenjin 1 ; Wang, Peng 2 ; Liu, Zhengguo 1 ;
作者机构： 1.Guangxi Univ, Coll Agr, Nanning 530004, Guangxi, Peoples R China
2.Guangxi Acad Agr Sci, Inst Vegetables, Nanning 530004, Peoples R China
关键词： Wax gourd; Genetic map; Whole-genome resequencing; Fruit-related trait; Quantitative trait loci
期刊名称：EUPHYTICA （ 影响因子：2.185； 五年影响因子：2.387 ）
ISSN： 0014-2336
年卷期： 2022 年 218 卷 8 期
页码：
收录情况： SCI
摘要： Wax gourd (Benincasa hispida [Thunb] Cogn.) is an important horticultural crop in the Cucurbitaceae family that has high nutritive and medicinal value. We analysed quantitative trait loci (QTLs) related to the fruit-related traits of wax gourd by constructing a high-density genetic map through whole-genome resequencing. We obtained an F-2 population of 195 individuals by crossing two inbred wax gourd lines: XDJQ-1 and SL-7. A high-density genetic map was constructed after generating a sequencing library and identifying and genotyping single-nucleotide polymorphisms (SNPs). Finally, we used QTL mapping to analyse the fruit-related traits of wax gourd. The high-density genetic map showed sufficient coverage to detect recombination breakpoints and contained 323,107 SNP markers, spanned 1243.87 cM, and had an average distance between adjacent markers of 0.33 cM. Furthermore, 3,720 bin markers (including SNP markers) were mapped to 12 linkage groups. Based on the high-density genetic map, one major QTL for fruit flesh colour (fc5.1) was mapped on the 3.379 Mb region of chromosome 5, and two major QTLs for fruit length (fl2.1) and fruit width (fw2.1) were mapped on the (0.345 Mb) and (0.602 Mb) regions of chromosome 2 respectively. The QTLs for fc5.1, fl2.1, and fw2.1 accounted for 28.684%, 63.562%, and 59.979% of wax gourd phenotypic variation, respectively. To our knowledge, ours is the first high-density genetic map of wax gourd constructed using whole-genome resequencing and is the highest density genetic map available for wax gourd. Our results provide a foundation for future map-based cloning, candidate gene identification, and marker-assisted breeding.
- 相关文献
作者其他论文 更多>>
-
Four closely related endornaviruses each with a low incidence in the phytopathogenic fungi Exserohilum turcicum or Bipolaris maydis
作者：Wang, Peng;Zheng, Yun;Pan, Xin;Gao, Zhongnan;Zhou, Xuan;Deng, Qingchao;Fang, Shouguo;Wang, Haoran;Zhang, Songbai;Pan, Shouhui;Dai, Fei;Li, Zhanbiao
关键词：Endornavirus; Mycovirus; Exserohilum turcicum; Bipolaris maydis; Vegetative incompatibility
-
Characteristics, Comparative Analysis, and Phylogenetic Relationships of Chloroplast Genomes of Cultivars and Wild Relatives of Eggplant (Solanum melongena)
作者：Yang, Qihong;Cai, Liangyu;Gan, Guiyun;Wang, Peng;Li, Weiliu;Li, Wenjia;Jiang, Yaqin;Li, Dandan;Wang, Mila;Chen, Riyuan;Wang, Yikui;Li, Ye;Wang, Mila;Chen, Riyuan;Xiong, Cheng
关键词：Solanum melongena; chloroplast genome; DNA barcode; comparative analysis; phylogenetic; interspecific hybridization
-
Development and Application of InDel Markers Linked to Fruit-Shape and Peel-Colour Genes in Wax Gourd
作者：Huang, Xiaochun;Wu, Wenting;Su, Liwen;Lv, Haixuan;Cheng, Zhikui;Yang, Wenrui;Nong, Lifeng;Liu, Ting;Liu, Zhengguo;Chen, Yong;Wang, Peng
关键词：fruit shape; gene; InDel marker; peel colour; wax gourd
-
Fine Mapping and Functional Analysis of Major Regulatory Genes of Soluble Solids Content in Wax Gourd (Benincasa hispida)
作者：Wu, Wenting;Huang, Xiaochun;Su, Liwen;Lv, Haixuan;Gou, Jiquan;Cheng, Zhikui;Ma, Lianlian;Yu, Wenjin;Liu, Zhengguo;Wang, Peng
关键词：wax gourd; soluble solids content; BSA; QTL; NADP-dependent malic enzyme
-
Comparative full-length transcriptome analysis by Oxford Nanopore Technologies reveals genes involved in anthocyanin accumulation in storage roots of sweet potatoes (Ipomoea batatas L.)
作者：Xiong, Jun;Yu, Wenjin;Xiong, Jun;Tang, Xiuhua;Wei, Minzheng
关键词：Sweet potato; Oxford Nanopore Technologies; Full-length transcriptome; Anthocyanin; Storage roots
-
Fine mapping and identification of the candidate gene BFS for fruit shape in wax gourd (Benincasa hispida)
作者：Cheng, Zhikui;Liu, Zhengguo;Ma, Lianlian;Chen, Jieying;Gou, Jiquan;Su, Liwen;Wu, Wenting;Yu, Wenjin;Wang, Peng;Xu, Yuanchao;Wang, Peng;Chen, Yong
关键词：
-
Identification and Application of BhAPRR2 Controlling Peel Colour in Wax Gourd (Benincasa hispida)
作者：Ma, Lianlian;Liu, Zhengguo;Cheng, Zhikui;Gou, Jiquan;Chen, Jieying;Yu, Wenjin;Wang, Peng;Wang, Peng
关键词：APRR2; chlorophyll; frameshift mutation; peel color; wax gourd
