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Genome-wide characterization and expression analysis of alpha-amylase and beta-amylase genes underlying drought tolerance in cassava

文献类型： 外文期刊

作者： Yang, Taiyi 1 ; Li, Hengrui 2 ; Li, Liangwu 1 ; Wei, Wanling 2 ; Huang, Yuanhang 1 ; Xiong, Faqian 3 ; Wei, Maogui 1 ;

作者机构： 1.Guangxi Univ, Coll Agron, Nanning 530004, Peoples R China

2.Guangxi South Subtrop Agr Sci Res Inst, Chongzuo 532406, Peoples R China

3.Guangxi Acad Agr Sci, Sugarcane Res Inst, Nanning 530007, Peoples R China

4.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Nanning 530004, Peoples R China

5.Guangxi Univ, Guangxi Key Lab Agroenvironm & Agroprod Safety, Nanning 530004, Guangxi, Peoples R China

关键词： Cassava; Amylase; Abiotic stress; Bio-informatics; Gene expression

期刊名称：BMC GENOMICS （ 影响因子：4.4； 五年影响因子：4.7 ）

ISSN： 1471-2164

年卷期： 2023 年 24 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundStarch hydrolysates are energy sources for plant growth and development, regulate osmotic pressure and transmit signals in response to both biological and abiotic stresses. The alpha-amylase (AMY) and the beta-amylase (BAM) are important enzymes that catalyze the hydrolysis of plant starch. Cassava (Manihot esculenta Crantz) is treated as one of the most drought-tolerant crops. However, the mechanisms of how AMY and BAM respond to drought in cassava are still unknown.ResultsSix MeAMY genes and ten MeBAM genes were identified and characterized in the cassava genome. Both MeAMY and MeBAM gene families contain four genes with alternative splicing. Tandem and fragment replications play important roles in the amplification of MeAMY and MeBAM genes. Both MeBAM5 and MeBAM10 have a BZR1/BES1 domain at the N-terminus, which may have transcription factor functions. The promoter regions of MeAMY and MeBAM genes contain a large number of cis-acting elements related to abiotic stress. MeAMY1, MeAMY2, MeAMY5, and MeBAM3 are proven as critical genes in response to drought stress according to their expression patterns under drought. The starch content, soluble sugar content, and amylase activity were significantly altered in cassava under different levels of drought stress.ConclusionsThese results provide fundamental knowledge for not only further exploring the starch metabolism functions of cassava under drought stress but also offering new perspectives for understanding the mechanism of how cassava survives and develops under drought.

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