Cultivation of Lactobacillus casei LT-L614 in High Density with Sugarcane Molasses: Optimization of Processing
文献类型： 外文期刊
作者： Peng, Juan 1 ; You, Qianqian 1 ; Feng, Bin 2 ; Chen, Zhineng 2 ; Lie, Yonghong 2 ; Tang, Zhiguang 1 ; Li, Xin 1 ; Wang, L 1 ;
作者机构： 1.Guangxi Univ, Coll Light Ind & Food Engn, Nanning 530004, Peoples R China
2.Guangxi Guangye Guitang Sugar Grp Co Ltd, Guigang 537102, Peoples R China
3.Collaborat Innovat Ctr Guangxi Sugar Ind, Nanning 530004, Peoples R China
4.SUNY Syracuse, Coll Environm Sci & Forestry, Syracuse, NY 13210 USA
5.Guangxi Acad Agr Sci, Sugarcane Res Inst, Nanning 530007, Peoples R China
6.Minist Agr, Key Lab Sugarcane Biotechnol & Genet Improvement, Nanning 530007, Peoples R China
7.Guangxi Key Lab Sugarcane Genet Improvement, Nanning 530007, Peoples R China
8.Chinese Acad Agr Sci, Sugarcane Res Ctr, Nanning 530007, Peoples R China
关键词： Sugarcane Molasses; Lactobacillus casei LT-L614; High Density Culture; Logistic Equations
期刊名称：JOURNAL OF BIOBASED MATERIALS AND BIOENERGY （ 影响因子：0.708； 五年影响因子：0.739 ）
ISSN： 1556-6560
年卷期： 2019 年 13 卷 6 期
页码：
收录情况： SCI
摘要： The culture medium was optimized based on the MRS medium by single factor tests and response surface technology. Specifically, the recipe was as follows: molasses of 80 g/L, soybean peptone of 10 g/L, yeast powder of 15 g/L, corn juice of 70 mL/L, sodium citrate of 8.5 g/L. The culture conditions were also optimized as initial pH of 6.9, temperature of 37 degrees C, and inoculation quantity of 2%. The combination of neutralization with intermittent feeding was applied for the fermentation. Moreover, a dynamic model was proposed for the cell growth of Lactobacillus casei LT-L614, through fitting reducing sugar amount and fermentation time. The feeding time interval was optimized according to the logistic model. It resulted in an optimal viable count of 9 x 12 x 10(11) cfu/mL, which was increased by two orders for magnitude compared to that without optimization.
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