Role of Phospholipase D Inhibitor in Regulating Expression of Senescence-related Phospholipase D Gene in Postharvest Longan Fruit
文献类型： 外文期刊
作者： Li, Li 1 ; Li, Jiemin 1 ; Sun, Jian 1 ; Yi, Ping 1 ; Li, Changbao 1 ; Zhou, Zhugui 1 ; Xin, Ming 1 ; Sheng, Jinfeng 1 ; Shua 1 ;
作者机构： 1.Guangxi Acad Agr Sci, Agrofood Sci & Technol Res Inst, 174 East Daxue Rd, Nanning 530007, Peoples R China
2.Guangxi Key Lab Fruits & Vegetables Storage Proc, 174 East Daxue Rd, Nanning 530007, Peoples R China
关键词： Phospholipase D; postharvest longan; 2-butanol; senescence; gene expression; inhibitor
期刊名称：CURRENT BIOINFORMATICS （ 影响因子：3.543； 五年影响因子：2.048 ）
ISSN： 1574-8936
年卷期： 2019 年 14 卷 7 期
页码：
收录情况： SCI
摘要： Background: Phospholipase D (PLD)is closely related to browning and senescence of postharvest longan fruit. Objective: This study investigated the effects of 2-butanol (a PLD inhibitor) on the expression and regulation of PLD during storage of longan fruit at a low temperature. Methods: Senescence-related quality indices showed that the 2-butanol-treated fruit presented lower pericarp browning index, pulp breakdown index and total soluble solid value than the untreated fruit. Results: The fruit treated by 60 mu L/L 2-butanol exhibited the strongest inhibition on senescence, which significantly delayed changes in weight, titratable acidity content, total soluble solid content and ascorbic acid content. This treatment maintained a high level of total phenolic content and caused significant inhibition on pericarp browning and pulp breakdown. Through ELISA method, 60 mu L/L, 2-butanol treatment also reduced PLD activity. Real-time RT-PCR (RT-qPCR) results showed that PLD mRNA expression level was inhibited by 60 mu L/L 2-butanol within 15 days. Western-blotting results further confirmed the differential expression of PLD during storage, and a relatively higher expression for PLD protein was found in control compared to the 2-butanoltreated fruit during 15-d storage. Conclusion: These results provided a scientific basis and reference to further investigating postharvest longan quality maintenance by regulating the PLD gene expression.
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