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Rapid improvement of grain appearance in three-line hybrid rice via CRISPR/Cas9 editing of grain size genes

文献类型： 外文期刊

作者： Huang, Juan 1 ; Chen, Weiwei 1 ; Gao, Lijun 2 ; Qing, Dongjin 1 ; Pan, Yinghua 1 ; Zhou, Weiyong 1 ; Wu, Hao 2 ; Li, Jingcheng 1 ; Ma, Chonglie 2 ; Zhu, Changlan 3 ; Dai, Gaoxing 1 ; Deng, Guofu 1 ;

作者机构： 1.Guangxi Acad Agr Sci, Rice Res Inst, Guangxi Key Lab Rice Genet & Breeding, Nanning 530007, Peoples R China

2.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China

3.Jiangxi Agr Univ, Key Lab Crop Physiol Ecol & Genet Breeding, Minist Educ, Nanchang 330045, Peoples R China

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：4.4； 五年影响因子：5.0 ）

ISSN： 0040-5752

年卷期： 2024 年 137 卷 7 期

页码：

收录情况： SCI

摘要： Key messageGenetic editing of grain size genes quickly improves three-line hybrid rice parents to increase the appearance quality and yield of hybrid rice.AbstractGrain size affects rice yield and quality. In this study, we used CRISPR/Cas9 to edit the grain size gene GW8 in the maintainer line WaitaiB (WTB) and restorer line Guanghui998 (GH998). The new slender sterile line WTEA (gw8) was obtained in the BC2F1 generation by transferring the grain mutation of the maintainer plant to the corresponding sterile line WantaiA (WTA, GW8) in the T1 generation. Two slender restorer lines, GH998E1 (gw8(II)) and GH998E2 (gw8(I)), were obtained in T1 generation. In the early stage, new sterile and restorer lines in grain mutations were created by targeted editing of GS3, TGW3, and GW8 genes. These parental lines were mated to detect the impact of grain-type mutations on hybrid rice yield and quality. Mutations in gs3, gw8, and tgw3 had a minimal impact on agronomic traits except the grain size and thousand-grain weight. The decrease in grain width in the combination mainly came from gw8/gw8, gs3/gs3 increased the grain length, gs3/gs3-gw8/gw8 had a more significant effect on the grain length, and gs3/gs3-gw8/gw8(I) contributed more to grain length than gs3/gs3-gw8/gw8(II). The heterozygous TGW3/tgw3 may not significantly increase grain length. Electron microscopy revealed that the low-chalky slender-grain variety had a cylindrical grain shape, a uniform distribution of endosperm cells, and tightly arranged starch grains. Quantitative fluorescence analysis of endospermdevelopment-related genes showed that the combination of slender grain hybrid rice caused by gs3 and gw8 mutations promoted endosperm development and improved appearance quality. An appropriate grain size mutation resulted in hybrid rice varieties with high yield and quality.

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