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In vitro antifungal activity of dimethyl trisulfide against Colletotrichum gloeosporioides from mango

文献类型： 外文期刊

作者： Tang, Lihua 1 ; Mo, Jianyou 1 ; Guo, Tangxun 1 ; Huang, Suiping 1 ; Li, Qili 1 ; Ning, Ping 3 ; Hsiang, Tom 4 ;

作者机构： 1.Guangxi Acad Agr Sci, Inst Plant Protect, Nanning 530007, Guangxi, Peoples R China

2.Key Lab Biol Crop Dis & Insect Pests Guangxi, Nanning 530007, Guangxi, Peoples R China

3.Guangxi Agr Vocat Coll, Nanning 530007, Guangxi, Peoples R China

4.Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada

关键词： Mango; Colletotrichum gloeosporioides; Dimethyl trisulfide; Antifungal activity

期刊名称：WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY （ 影响因子：3.312； 五年影响因子：3.58 ）

ISSN： 0959-3993

年卷期： 2020 年 36 卷 1 期

页码：

收录情况： SCI

摘要： Colletotrichum gloeosporioides, one of the main agents of mango anthracnose, causes latent infections in unripe mango, and leads to huge economic losses during storage and transport. Dimethyl trisulfide (DMTS), one of the main volatile compounds produced by some microorganisms or plants, has shown antifungal activity against some phytopathogens in previous studies, but its effects on C. gloeosporioides and mechanisms of action have not been well characterized. In fumigation trials of conidia and mycelia of C. gloeosporioides for 2, 4, 6, 8, or 10 h, at a concentration of 100 mu L/L of air space in vitro, DMTS caused serious damage to the integrity of plasma membranes, which significantly reduced the survival rate of spores, and resulted in abnormal hyphal morphology. Moreover, DMTS caused deterioration of subcellular structures of conidia and mycelia, such as cell walls, plasma membranes, Golgi bodies, and mitochondria, and contributed to leakage of protoplasm, thus promoting vacuole formation. In addition, to better understand the molecular mechanisms of the antifungal activity, the global gene expression profiles of isolate C. gloeosporioides TD3 treated in vitro with DMTS at a concentration of 100 mu L/L of air for 0 h (Control), 1 h, or 3 h were investigated by RNA sequencing (RNA-seq), and over 62 Gb clean reads were generated from nine samples. Similar expressional patterns for nine differentially expressed genes (DEGs) in both RNA-seq and qRT-PCR assays showed the reliability of the RNA-seq data. In comparison to the non-treated control groups, we found DMTS suppressed expression of beta-1, 3-d-glucan, chitin, sterol biosynthesis-related genes, and membrane protein-related genes. These genes related to the formation of fungal cell walls and plasma membranes might be associated with the toxicity of DMTS against C. gloeosporioides. This is the first study demonstrating antifungal activity of DMTS against C. gloeosporioides on mango by direct damage of conidia and hyphae, thus providing a novel tool for postharvest control of mango anthracnose.

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