Full-length agave transcriptome reveals candidate glycosyltransferase genes involved in hemicellulose biosynthesis
文献类型： 外文期刊
作者： Huang, Xing 1 ; Hu, Xiaoli 2 ; Liu, Qingqing 3 ; Xie, Zhouli 2 ; Tan, Shibei 1 ; Qin, Xu 4 ; Chen, Tao 4 ; Wu, Weihuai 1 ; Saud, Shah 5 ; Nawaz, Taufiq 6 ; El-Kahtany, Khaled 7 ; Fahad, Shah 6 ; Yi, Kexian 9 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Natl Key Lab Trop Crop Breeding, Haikou 571101, Peoples R China
2.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Peoples R China
3.Shanghai Chenshan Bot Garden, Shanghai Key Lab Plant Funct Genom & Resources, Shanghai 201602, Peoples R China
4.Guangxi Subtrop Crops Res Inst, Nanning 530001, Peoples R China
5.Linyi Univ, Coll Life Sci, Linyi 276000, Shandong, Peoples R China
6.South Dakota State Univ, Dept Biol & Microbiol, Brookings, SD 57007 USA
7.Abdul Wali Khan Univ Mardan, Dept Agron, Khyber Pakh Tunkhwa 23200, Pakistan
8.King Saud Univ, Coll Sci, Geol & Geophys Dept, POB 2455, Riyadh 11451, Saudi Arabia
9.Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya 572025, Peoples R China
10.Minist Agr & Rural Affairs, Key Lab Integrated Pest Management Trop Crops, Haikou 571101, Hainan, Peoples R China
11.Hainan Key Lab Monitoring & Control Trop Agr Pests, Haikou 571101, Peoples R China
关键词： Agave; Full-length transcriptome; Glycosyltransferase gene; Hemicellulose biosynthesis; Leaf development; Fungus infection
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：7.7； 五年影响因子：7.7 ）
ISSN： 0141-8130
年卷期： 2024 年 274 卷
页码：
收录情况： SCI
摘要： Agave species are typical crassulacean acid metabolism (CAM) plants commonly cultivated to produce beverages, fibers, and medicines. To date, few studies have examined hemicellulose biosynthesis in Agave H11648, which is the primary cultivar used for fiber production. We conducted PacBio sequencing to obtain full-length transcriptome of five agave tissues: leaves, shoots, roots, flowers, and fruits. A total of 41,807 genes were generated, with a mean length of 2394 bp and an annotation rate of 97.12 % using public databases. We identified 42 glycosyltransferase genes related to hemicellulose biosynthesis, including mixed-linkage glucan (1), glucomannan (5), xyloglucan (16), and xylan (20). Their expression patterns were examined during leaf development and fungal infection, together with hemicellulose content. The results revealed four candidate glycosyltransferase genes involved in xyloglucan and xylan biosynthesis, including glucan synthase (CSLC), xylosyl transferase (XXT), xylan glucuronyltransferase (GUX), and xylan alpha-1,3-arabinosyltransferase (XAT). These genes can be potential targets for manipulating xyloglucan and xylan traits in agaves, and can also be used as candidate enzymatic tools for enzyme engineering. We have provided the first full-length transcriptome of agave, which will be a useful resource for gene identification and characterization in agave species. We also elucidated the hemicellulose biosynthesis machinery, which will benefit future studies on hemicellulose traits in agave.
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