Overexpression of alpha-Tubulin Gene of Sugarcane (Saccharum spp. hybrids), SoTUA, Enhances Tobacco Tolerance to Cold Stress
文献类型： 外文期刊
作者： Chen, Jiao-Yun 3 ; Sun, Bo 4 ; Khan, Qaisar 1 ; Yang, Li-Tao 1 ; Zhang, Bao-Qing 2 ; Xing, Yong-Xiu 1 ; Li, Yang-Rui 2 ;
作者机构： 1.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China
2.Chinese Acad Agr Sci, Sugarcane Res Inst, Guangxi Acad Agr Sci,Key Lab Sugarcane Biotechnol, Sugarcane Res Ctr,Minist Agr & Rural Affairs,Guan, Nanning 530007, Peoples R China
3.South China Agr Univ, Coll Agr, Guangzhou 510640, Peoples R China
4.Duke Univ, Sch Med, Div Cardiol, Dept Pediat, Durham, NC 27710 USA
关键词： Tobacco; SoTUA; Transformation; Cold stress; Sugarcane
期刊名称：SUGAR TECH （ 影响因子：1.872； 五年影响因子：1.873 ）
ISSN： 0972-1525
年卷期：
页码：
收录情况： SCI
摘要： Low temperature stress affects the yield and quality of crops. To adapt to abiotic stress, plant tubulin families play a crucial role to increase cell cold stability. In the present study, our aim was to develop transgenic tobacco by using a cold-responsive SoTUA gene isolated from Saccharum spp. hybrid. Based on fluorescent microscope observation and TUA gene expression analysis in different tissues, TUA gene expression was found mainly in veins. Moreover, a significant increase of TUA transcripts and protein under cold stress was detected by quantificational real-time polymerase chain reaction (qRT-PCR) and western blot in the transgenic plants. Results of peroxidase (POD), superoxide dismutase (SOD) and catalase (CAT) activities, and proline, soluble sugar and protein contents indicated oxidative damage is less than wild type (WT). Overexpression of SoTUA gene has improved the cold stress tolerance ability in tobacco.
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