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Validation of Interaction Between Sugarcane miR167d and Predicted Target Gene ScPUM1 and Analysis of Their Expression Under Cold Stress: A Novel Mechanism of miR167d

文献类型： 外文期刊

作者： Song, Qiqi 1 ; Zhu, Pengjin 1 ; Li, Jiahui 1 ; Cheng, Qin 1 ; Tan, Qinliang 1 ; Zhou, Quanguang 1 ; Lv, Ping 1 ; Nong, Zemei 1 ;

作者机构： 1.Guangxi Subtrop Crops Res Inst, Nanning 530009, Guangxi, Peoples R China

2.Minist Agr & Rural Affairs, Key Lab Qual & Safety Control Subtrop Fruit & Vege, Nanning 530009, Guangxi, Peoples R China

关键词： Sugarcane; Cold stress; MiR167d; ScPUM1

期刊名称：JOURNAL OF PLANT GROWTH REGULATION （ 影响因子：4.4； 五年影响因子：4.9 ）

ISSN： 0721-7595

年卷期： 2025 年 44 卷 5 期

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs) serve as crucial endogenous small molecules in plants, functioning through the regulation of target gene expression. Previous studies by our research group discovered that miR167d responds to cold stress in sugarcane. Current reports on the target genes of miR167d predominantly focus on the ARF gene family, with no validation reports on other target genes. Utilizing the RNA22 online software, we predicted pumilio homolog 1 (PUM1) as a potential target gene of miR167d. This study fused the target gene with the GUS reporter gene and validated the interaction between miR167d and ScPUM1 through Agrobacterium-mediated transient co-transformation in tobacco. The cleavage sites of miR167d on ScPUM1 were identified using the 5 ' RACE technique. Subcellular localization revealed that ScPUM1 functions in the endoplasmic reticulum. RT-qPCR was employed to monitor the expression levels of miR167d and the ScPUM1 gene in sugarcane leaves after 0, 2, 6, 12, and 24 h of exposure to 4 degrees C cold stress. It was observed that under cold conditions, the expression of miR167d dynamically changed with the duration of stress, with an increase in ScPUM1 expression after 12 h of stress when the expression of miR167d decreased. However, the overall regulatory effect of miR167d on ScPUM1 was minimal. We hypothesize that miR167d mediates the expression of target genes on the endoplasmic reticulum by cleaving ScPUM1, and that other pathways may also be involved in the regulation of key sites under cold stress. This study provides new insights into the complex regulatory network of sugarcane's response to cold stress.

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