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Exploring cadmium stress responses in sisal roots: Insights from biochemical and transcriptome analysis

文献类型： 外文期刊

作者： Li, Jing 1 ; Ge, Lifang 2 ; Liu, Ping 1 ; Huang, Zhaoxue 1 ; Tan, Shibei 2 ; Wu, Weihuai 2 ; Chen, Tao 3 ; Xi, Jingen 2 ; Huang, Xing 2 ; Yi, Kexian 1 ; Chen, Helong 1 ;

作者机构： 1.Guizhou Univ, Natl Key Lab Green Pesticide, Key Lab Green Pesticide & Agr Bioengn, Minist Educ,Ctr R&D Fine Chem, Guiyang, Peoples R China

2.Chinese Acad Trop Agr Sci CATAS, Environm & Plant Protect Inst, Haikou, Hainan, Peoples R China

3.Guangxi Subtrop Crops Res Inst, Nanning, Peoples R China

4.Chinese Acad Trop Agr Sci, Sanya Res Inst, Haikou, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.7； 五年影响因子：3.8 ）

ISSN： 1932-6203

年卷期： 2023 年 18 卷 11 期

页码：

收录情况： SCI

摘要： Sisal is a leaf fiber crop with a high integrated value and a wide range of uses in the application of soil remediation of heavy metal contamination. This study provides a preliminary understanding of how sisal responds to Cd stress and presents a theoretical basis for exploring the potential of sisal in the remediation of Cd-contaminated soils. In this work, the activities of the antioxidant enzymes (SOD, POD, and CAT) of sisal were measured by hydroponics with the addition of CdCl2 center dot 2.5H2O and different concentrations of Cd stress. Whole transcriptome sequencing (RNA-Seq) analysis was performed with lllumina sequencing technology, and qRT-PCR was conducted to verify the differential genes. The results obtained were as follows: (1) Short-term low concentration of Cd stress (20 mg/kg) had a transient promotion effect on the growth of sisal roots, but Cd showed a significant inhibitory effect on the growth of sisal roots over time. (2) Under different concentrations of Cd stress, the Cd content in sisal root was greater than that in sisal leaf, and Cd accumulated mainly in sisal roots. (3) With the increase of Cd stress concentration, the antioxidant enzyme catalase activity increased, peroxidase activity showed a decreasing trend, and superoxide dismutase showed a trend of increasing and then decreasing. (4) Transcriptome sequencing analysis detected 123 differentially expressed genes (DEGs), among which 85 genes were up-regulated and 38 genes were down-regulated. The DEGs were mainly concentrated in flavonoid biosynthesis and glutathione metabolism, and both processes had some regulatory effects on the Cd tolerance characteristics of sisal. This study elucidated the physiological, biochemical and transcriptomic responses of sisal under cadmium stress, and provided a theoretical basis for the ecological restoration function of sisal.

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