Identification of Ethylene Responsive miRNAs and Their Targets from Newly Harvested Banana Fruits Using High-Throughput Sequencing
文献类型： 外文期刊
作者： Dan, Ming 1 ; Huang, Meihua 1 ; Liao, Fen 1 ; Qin, Renyuan 1 ; Liang, Xiaojun 1 ; Zhang, Ezhen 1 ; Huang, Maokang 1 ; Hua 1 ;
作者机构： 1.Guangxi Acad Agr Sci, Inst Agrofood Sci & Technol, 174 East Daxue Rd, Nanning 530007, Peoples R China
2.Guangxi Crop Genet Improvement Lab, Nanning 530007, Peoples R China
3.Guangxi Key Lab Fruits & Vegetables Storage Proc, Nanning 530007, Peoples R China
关键词： microRNA; banana fruit; newly harvested; ethylene response; high-throughput sequencing
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）
ISSN： 0021-8561
年卷期： 2018 年 66 卷 40 期
页码：
收录情况： SCI
摘要： The roles of microRNAs (miRNAs) related to ethylene response in banana fruits remain unknown because many miRNAs are differentially expressed as the fruit ripens, making the identification of ethylene-responsive miRNAs difficult. Using newly harvested banana fruits (within 5 h after harvest) as material, we found that these fruit did not ripen when treated with 5 mu L/L of ethylene for 12 h at 22 degrees C. Two miRNA libraries were generated from newly harvested banana fruits with and without ethylene treatment and sequenced. In total, 128 known miRNAs belonging to 42 miRNA families were obtained, and 12 novel miRNAs were identified. Among them, 22 were differentially expressed in response to ethylene treatment, among which 6 known miRNAs and their putative targets were validated using qRT-PCR These putative targets encoded proteins including GATA, ARF, DLC, and AGO, etc. KEGG and GO analyses showed that miRNAs differentially expressed in response to ethylene mainly function in the molecular and biological processes.
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