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Molecular Cloning and Expressional Analysis of Five Sucrose Transporter (SUT) Genes in Sugarcane

文献类型： 外文期刊

作者： Niu, Jun-Qi 1 ; Huang, Jing-Li 1 ; Thi-Thu Phan 1 ; Pan, Yong-Bao 3 ; Yang, Li-Tao 1 ; Li, Yang-Rui 2 ;

作者机构： 1.Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobi, 100 East Daxue Rd, Nanning 530005, Peoples R China

2.Chinese Acad Agr Sci, Key Lab Sugarcane Biotechnol & Genet Improvement, Guangxi Key Lab Sugarcane Genet Improvement,China, Guangxi Acad Agr Sci,Sugarcane Res Inst,Sugarcane, Nanning 530007, Peoples R China

3.USDA ARS, Southeast Area, Sugarcane Res Unit, Houma, LA 70360 USA

关键词： Sugarcane; Sucrose transporter (SUT); Gene expression; Sucrose accumulation

期刊名称：SUGAR TECH （ 影响因子：1.591； 五年影响因子：1.688 ）

ISSN： 0972-1525

年卷期： 2019 年 21 卷 1 期

页码：

收录情况： SCI

摘要： Five SUT genes, namely SoSUT1, SoSUT2, SoSUT3, SoSUT4, and SoSUT5, were cloned, and their expressions in roots, stems, leaves, inflorescence, and buds at physiological mature stage were analyzed by qRT-PCR. Major findings of the study were: (1) the molecular mass of deduced sugarcane sucrose transporter proteins was between 53.44 and 61.80kDa, and the pIs were between 5.94 and 10.68; (2) all sugarcane sucrose transporter proteins had 12 typical transmembrane domains; (3) there were two DNA sequences encoding the SoSUT3 gene with six exons and five introns; (4) while SoSUT2, SoSUT4, and SoSUT5 belonged to Clades SUT2, SUT4, and SUT5, other SoSUT proteins belonged to Clade SUT3; (5) real-time PCR analyses results showed SoSUT1, SoSUT4, and SoSUT5 had highly abundant expression in the inflorescence, and SoSUT2 and SoSUT3 highly expressed in the buds and inflorescence at physiological maturity. These data may indicate that different SUT genes may be transcribed during the sucrose accumulation process in sugarcane stems and that the expressions of SoSUTs may play an important role in sugarcane inflorescence development.

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