文献类型： 外文期刊
作者： Jin, Lian 2 ; Zhang, Chuanxiang 4 ; Deng, Yan 3 ; Jia, Qi 2 ; Liu, Yuan 3 ; He, Nongyue 1 ; Wang, Lijun 5 ; Weng, Menglin 1 ;
作者机构： 1.Southeast Univ, Sch Biol Sci & Med Engn, Natl Demonstrat Ctr Expt Biomed Engn Educ, State Key Lab Bioelect, Nanjing 210096, Jiangsu, Peoples R China
2.Zunyi Med Univ, Bioresource Inst Hlth Utilizat, Zunyi 563000, Guizhou, Peoples R China
3.Hunan Univ Technol, Econ Forest Cultivat & Utilizat Collaborat Innova, Hunan Key Lab Biomed Nanomat & Devices, Zhuzhou 412007, Peoples R China
4.Hunan Univ Technol, Sch Packaging & Mat Engn, Zhuzhou 412007, Hunan, Peoples R China
5.Guangxi Univ, Coll Light Ind & Food Engn, Nanning 530004, Peoples R China
6.Guangxi Key Lab Clean Pulp & Paper Making & Pollu, Nanning 530007, Peoples R China
7.Guangxi Sugarcane Res Inst, Nanning 530007, Peoples R China
关键词： Polyethylenimine; Chitosan; Gene Delivery; N/P Ratio; Efficiency
期刊名称：NANOSCIENCE AND NANOTECHNOLOGY LETTERS （ 影响因子：1.128； 五年影响因子：0.985 ）
ISSN： 1941-4900
年卷期： 2018 年 10 卷 7 期
页码：
收录情况： SCI
摘要： Gene delivery, as one critical component of gene therapy, has been widely investigated to develop reliable carriers for efficient gene transfer. Among the mounting carriers, polymers such as polyethylenimine and chitosan attracted considerable attention due to their good biocompatibility and relatively high gene delivery efficiency. In this study, polyethylenimine was utilized as the carrier for plasmid DNA to cells and achieved transfection efficiency as high as 50-60% with the optimized N/P ratio (ratio of nitrogen of polymer to phosphate of DNA) in the range from 6:1 to 3:1. The presence of serum did not affect the gene delivery efficiency of polyethylenimine. However, neither chitosan nor chitosan nanoparticles showed high activity in transferring plasmid DNA into cells. More efforts on the optimization of chitosan properties are warranted to make chitosan a promising carrier for gene delivery. In conclusion, polyethylenimine-based nanoparticles are of great potential in gene delivery and chitosan could become a promising gene delivery carrier with appropriate modifications.
- 相关文献
作者其他论文 更多>>
-
Low ammonium and high nitrate input improves nitrogen use efficiency and growth in sugarcane through coordinated reprogramming of nitrogen and carbon metabolism
作者：Zhan, Jian;Zhou, Yifan;Yang, Linsheng;Deng, Ke;Zhang, Di;Meng, Bo;Wang, Huayang;Deng, Yan;Chen, Xinping;Zhang, Fusuo;Zhan, Jian;Zhou, Yifan;Yang, Linsheng;Deng, Ke;Zhang, Di;Meng, Bo;Wang, Huayang;Deng, Yan;Chen, Xinping;Zhan, Jian;Wang, Huayang;Deng, Yan;Chen, Xinping;Lakshmanan, Prakash;Lakshmanan, Prakash;Zhang, Fusuo
关键词：Ammonium-to-nitrate-ratio; Ammonium toxicity; Multi-omics; Nitrogen assimilation; Sugarcane
-
A naturally occurring SNP modulates thermotolerance divergence among grapevines
作者：Chen, Haiyang;Yu, Haibo;Kong, Lingchao;Li, Shenchang;Li, Yang;Wang, Yi;Duan, Wei;Dai, Zhanwu;Fan, Peige;Li, Shaohua;Liang, Zhenchang;Wang, Lijun;Chen, Haiyang;Yu, Haibo;Kong, Lingchao;Li, Shenchang;Li, Yang;Wang, Yi;Duan, Wei;Dai, Zhanwu;Fan, Peige;Li, Shaohua;Liang, Zhenchang;Wang, Lijun;Chen, Haiyang;Chen, Haiyang;Yu, Haibo;Kong, Lingchao;Li, Shenchang;Dai, Zhanwu;Fan, Peige;Liang, Zhenchang;Wang, Lijun;Yuan, Ling;Yuan, Ling;Cao, Xiongjun;Lin, Ling;Guo, Rongrong;Xie, Taili
关键词：
-
Bioinspired Heterogeneous Wettability Triboelectric Sensors for Sweat Collection and Monitoring
作者：Li, Xuedi;Liu, Tao;Yu, Kang;Zhang, Song;Ye, Ziyi;Wang, Jinlong;Liu, Yanhua;Cai, Chenchen;Chi, Mingchao;Luo, Bin;Wang, Lijun;Nie, Shuangxi;Weng, Mengling
关键词：cellulose; energy harvesting; sweat sensor; triboelectric materials; triboelectric nanogenerators
-
Environmental trade-offs and productivity gains through sustainable nitrogen use in an intensive high-input sugarcane production system in China
作者：Yang, Linsheng;Yang, Linsheng;Zhou, Yifang;Meng, Bo;Wang, Huayang;Lakshmanan, Prakash;Deng, Yan;Chen, Xinping;Zhou, Yifang;Liu, Xiaoyan;Lakshmanan, Prakash;Luo, Ting;Lakshmanan, Prakash;Zhang, Fusuo
关键词：Sugarcane; Nitrogen management; Yield; Reactive nitrogen loss
-
Development of InDel markers and construction of DNA fingerprints of wax gourd (Benincasa hispida) based on whole genome re-sequencing
作者：Su, Liwen;Cheng, Zhikui;Wu, Yongguan;Deng, Yan;Bai, Wenhui;Chen, Zhihao;Chen, Xianglei;Wang, Peng;Liu, Zhengguo;Li, Jingying;Li, Yan
关键词：Wax gourd; Whole genome re-sequencing; InDel marker; DNA fingerprints
-
Comparative metabolomics reveals complex metabolic shifts associated with nitrogen-induced color development in mature pepper fruit
作者：Zhang, Lu;Zhang, Fen;He, Xuanyi;Dong, Yuehua;Sun, Kai;Liu, Shunli;Wang, Xiaozhong;Yang, Huaiyu;Zhang, Wei;Lakshmanan, Prakash;Chen, Xinping;Deng, Yan;Zhang, Lu;Zhang, Fen;He, Xuanyi;Dong, Yuehua;Sun, Kai;Liu, Shunli;Wang, Xiaozhong;Chen, Xinping;Deng, Yan;Yang, Huaiyu;Zhang, Wei;Lakshmanan, Prakash;Lakshmanan, Prakash;Lakshmanan, Prakash
关键词：pepper; fruit color; nitrogen; carotenoids; flavonoids; metabolome
-
High sugarcane yield and large reduction in reactive nitrogen loss can be achieved by lowering nitrogen input
作者：Yang, Linsheng;Xi, Min;Wu, Wenge;Yang, Linsheng;Zhou, Yifang;Meng, Bo;Zhan, Jian;Deng, Yan;Lakshmanan, Prakash;Chen, Xinping;Lakshmanan, Prakash;Lakshmanan, Prakash;Zhang, Fusuo
关键词：N leaching; Ammonia loss; N2O emissions; N balance; Sugarcane
