您好，欢迎访问广西农业科学院 机构知识库！

Two Genes (ClS1 and ClF-box) Involved the Self-Incompatibility of "Xiangshui" Lemon (Citrus limon (L.) Burm. f.)

文献类型： 外文期刊

作者： Lin, Wei 1 ; Zhang, Shuwei 2 ; Ding, Feng 1 ; He, Xinhua 1 ; Luo, Cong 1 ; Huang, Guixiang 1 ; Do, Minhphu 1 ; Wang, Qing 1 ;

作者机构： 1.Guangxi Univ, Coll Agr, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Peoples R China

2.Guangxi Acad Agr Sci, Inst Hort Res, Nanning 530007, Peoples R China

3.South China Agr Univ, State Lab Conservat & Utilizat Subtrop Agrobiores, Guangzhou 510642, Guangdong, Peoples R China

关键词： Lemon; Self-incompatibility; S-RNase; SLF; Genetic transformation

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN： 0735-9640

年卷期： 2019 年 37 卷 1-2 期

页码：

收录情况： SCI

摘要： Gametophytic self-incompatibility (GSI) is an important factor affecting fruit development and is one of the important mechanisms of seedlessness in citrus. Although many studies have investigated the mechanisms of SI, this process remains unclear in Xiangshui lemon (C. limon). In this study, we cloned one S-RNase homologous gene and one SLF homologous gene from the Xiangshui lemon, designated as ClS1 (pistil-specific expression) and ClF-box (pollen-specific expression), which contain open reading frames (ORFs) of 534 and 810bp, respectively. The expression pattern of these two genes was analyzed by quantitative real-time polymerase chain reaction, and the results showed that the expression pattern of these two genes was significantly upregulated after self-pollination compared to that after cross-pollination. Through pollination in transgenic tobacco, we found that the pollen tubes grew slowly and became twisted after cross-pollination of ClS1f and ClF-box o transgenic tobaccos, but the pollen tubes of control, self-pollination ClS1 and cross-pollination ClS1o, and ClF-box f grew normally. Furthermore, significantly fewer pollen tubes of ClS1f and ClF-box o entered the ovules than that in other combinations, and the seed number of cross-pollination ClS1f and ClF-box o transgenic tobaccos was significantly reduced. These results indicated that the ClS1 and ClF-box genes contribute to the self-incompatibility of the Xiangshui lemon.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广西农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台