文献类型： 外文期刊
作者： Zhai, Yutong 1 ; Yu, Yonghao 1 ; Xu, Pengfei 1 ; Zeng, Xianru 1 ; Long, Xiuzhen 1 ; Wei, Dewei 1 ; He, Zhan 1 ; Gao, Xuyuan 1 ;
作者机构： 1.Guangxi Acad Agr Sci, Plant Protect Res Inst, Nanning 530007, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Green Prevent & Control Fruits & Vegetable, Nanning 530007, Peoples R China
3.Guangxi Key Lab Biol Crop Dis & Insect Pests, Nanning 530007, Peoples R China
关键词： reference genes; RT-qPCR; Bactrocera tau; different stages; different body parts
期刊名称：INSECTS （ 影响因子：2.9； 五年影响因子：3.3 ）
ISSN：
年卷期： 2025 年 16 卷 5 期
页码：
收录情况： SCI
摘要： The selection of appropriate reference genes is critical for standardizing quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) data, thereby ensuring accurate and reliable results of gene expression analysis. In this study, we identified 10 candidate reference genes (encoding alpha-tubulin, G6PDH, Rab1, RT, RPS13, beta-tubulin, DPH1, HSP90, GAPDH, and CP) and evaluated their suitability for use as reference genes in the pest insect, Bactrocera tau. Analysis was conducted using three software-based methods-Delta CT, NormFinder, and BestKeeper-alongside the online tool RefFinder. Expression levels of these genes were analyzed across various B. tau developmental stages and body parts. The overall ranking of reference gene stability scores was as follows: alpha-tubulin > G6PDH > CP > beta-tubulin > RT > HSP90 > GAPDH > DPH1 > RPS13 > Rab1. Ultimately, alpha-tubulin and G6PDH were identified as the most stable reference genes for B. tau.
- 相关文献
作者其他论文 更多>>
-
Research on the Expression of Immune-Related Genes at Different Stages in the Third-Instar Larvae of Spodoptera frugiperda Infected by Metarhizium rileyi
作者：Xu, Pengfei;He, Zhan;Gao, Xuyuan;Zeng, Xianru;Wei, Dewei;Long, Xiuzhen;Yu, Yonghao;Xu, Pengfei;He, Zhan;Gao, Xuyuan;Zeng, Xianru;Wei, Dewei;Long, Xiuzhen;Yu, Yonghao;Xu, Pengfei;He, Zhan;Gao, Xuyuan;Zeng, Xianru;Wei, Dewei;Long, Xiuzhen;Yu, Yonghao
关键词：
Metarhizium rileyi ;Spodoptera frugiperda ; infection process; innate immunity; RNA-seq -
Exploring the Life Cycle of Bactrocera latifrons: A Detailed Age-Stage, Two-Sex Life Table
作者：Zhai, Yutong;Zeng, Xianru;Wei, Dewei;Jiang, Xiaodong;Long, Xiuzhen;He, Zhan;Yu, Yonghao;Gao, Xuyuan;Zhai, Yutong;Zeng, Xianru;Wei, Dewei;Jiang, Xiaodong;Long, Xiuzhen;He, Zhan;Yu, Yonghao;Gao, Xuyuan;Zhai, Yutong;Zeng, Xianru;Wei, Dewei;Jiang, Xiaodong;Long, Xiuzhen;He, Zhan;Yu, Yonghao;Gao, Xuyuan
关键词：pepper fruit fly; developmental duration; male and female lifespan table; eggplant; group rearing
-
Symbiotic bacteria participate in pectinolytic metabolism to enhance larval growth in Zeugodacus cucurbitae
作者：Chen, Guangmei;Yue, Yang;Zheng, Dehong;Tan, Huihua;Chen, Guangmei;Tian, Zhenya;Yang, Jingfang;Zhou, Zhongshi;Gao, Xuyuan;Chen, Hongsong;Yang, Jingfang;Zhou, Zhongshi;Ma, Weihua
关键词：Zeugodacus cucurbitae; feeding wounds; Klebsiella; pectinases; pectinolytic metabolism
-
Electroantennographic and Behavioral Responses of the Melon fly, Zeugodacus cucurbitae (Coquillett), to Volatile Compounds of Ridge Gourd, Luffa acutangular L.
作者：Wang, Jing jing;Ma, Chao;Yang, Jin fang;Zhou, Zhong shi;Wang, Jing jing;Ma, Chao;Tian, Zhen ya;Zhou, Yong ping;Yang, Jin fang;Gao, Xuyuan;Chen, Hong song;Zhou, Zhong shi;Tian, Zhen ya;Zhou, Yong ping;Gao, Xuyuan;Chen, Hong song;Ma, Wei hua
关键词：Zeugodacus cucurbitae; GC-MS; Plant Volatiles; EAG; Behavioral Responses
-
Identification of candidate chemosensory genes in Bactrocera cucurbitae based on antennal transcriptome analysis
作者：Wang, Jing Jing;Ma, Chao;Yang, Jingfang;Wang, Yi Ting;Zhou, Zhongshi;Wang, Jing Jing;Ma, Chao;Yue, Yang;Yang, Jingfang;Chen, Li Xiang;Wang, Yi Ting;Gao, Xuyuan;Chen, Hong Song;Zhou, Zhongshi;Zhao, Chen Chen;Gao, Xuyuan;Chen, Hong Song;Ma, Wei Hua
关键词：Bactrocera cucurbitae; chemosensory genes; transcriptome; insect olfaction; pest management
-
Male-derived phospholipase A2 enhances WD46 expression and increases fertility in Ophraella communa
作者：Zhang, Yan;Zhang, Qinglu;Ma, Chao;Chen, Guangmei;Yue, Yang;Yang, Jingfang;Wan, Fanghao;Zhou, Zhongshi;Gao, Xuyuan;Zhou, Zhongshi
关键词：gene expression; PLA2; reproduction; seminal fluid protein; WD46
-
Emamectin benzoate-induced stress significantly affects the gut microbiome of adult Zeugodacus cucurbitae
作者：Li, Ruwen;Tian, Zhenya;Yang, Jingfang;Wang, Yusha;Zhou, Zhongshi;Li, Ruwen;Tian, Zhenya;Yang, Jingfang;Gao, Xuyuan;Chen, Hongsong;Wang, Yusha;Zhou, Zhongshi;Gao, Xuyuan;Chen, Hongsong;Wang, Yusha
关键词：Zeugodacus cucurbitae; Emamectin benzoate; Intestinal microorganism; Microbial diversity
