Integrated transcriptomic and proteomic analysis revealed the regulatory role of 5-azacytidine in kenaf salt stress alleviation
文献类型： 外文期刊
作者： Luo, Dengjie 1 ; Li, Zengqiang 1 ; Mubeen, Samavia 1 ; Rehman, Muzammal 1 ; Cao, Shan 1 ; Wang, Caijin 1 ; Yue, Jiao 1 ; Pan, Jiao 1 ; Jin, Gang 3 ; Li, Ru 2 ; Chen, Tao 3 ; Chen, Peng 1 ;
作者机构： 1.Guangxi Univ, Coll Agr, Key Lab Crop Genet Breeding & Germplasm Innovat, Guangxi Key Lab Agroenvironm & Agr Prod Safety, Nanning 530004, Peoples R China
2.Guangxi Univ, Coll Life Sci & Technol, Nanning 530004, Peoples R China
3.Guangxi Subtrop Crops Res Inst, Nanning 530001, Peoples R China
4.Henan Inst Sci & Technol, Henan Collaborat Innovat Ctr Modern Biol Breeding, Xinxiang 453003, Peoples R China
关键词： Kenaf; Salt stress; Transcriptomics; Proteomics; Correlation analysis
期刊名称：JOURNAL OF PROTEOMICS （ 影响因子：2.8； 五年影响因子：3.0 ）
ISSN： 1874-3919
年卷期： 2024 年 309 卷
页码：
收录情况： SCI
摘要： Salinity stress limits agricultural production. The DNA methyltransferase inhibitor, 5-azacitidine (5-azaC), plays a role in plant abiotic stress regulation, but its molecular basis in mediating salinity tolerance in kenaf remains unclear. To investigate the effects on 5-azaC on alleviating salt stress, kenaf seedlings were pre-treated with 0, 50, 100, 150, and 200 mu M 5-azaC and then exposed to 150 mM NaCl in a nutrient solution. Physiological, transcriptomic, and proteomic analyses were conducted on the root system to understand the regulatory mechanism of 5-azaC (comparing 5-azaC150 and control group 5-azaC0) under salt stress. The results indicated that 5-azaC significantly mitigated salt stress in kenaf by activating the antioxidant system, reducing reactive oxygen species (ROS), and increasing starch, soluble sugars, and adenosine triphosphate (ATP) content. A total of 14,348 differentially expressed genes (DEGs) and 313 differentially abundant proteins (DAPs) were identified. Combined proteomic and transcriptomic analysis revealed 27 DEGs/DAPs, with jointly up-regulated proteins (genes) including HcTHI1, HcBGLU11, and HcCBL1, and jointly down-regulated proteins (genes) including HcGAPDH, HcSS, and HcPP2C52. Overexpression and virus-induced gene silencing (VIGS) of HcPP2C52 demonstrated its role as a negative regulator of salt tolerance. These findings provide insights into the regulatory role of 5-azaC in plant responses to abiotic stresses. Significance: The specific molecular mechanism by which 5-azaC affects gene expression and protein activity of kenaf has been revealed, leading to enhanced salt tolerance.
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