Dual-electrode signal amplification self-powered biosensing platform based on nanozyme boosting target-induced DNA nanospace array for ultrasensitive detection of sugarcane Pokkah Boeng disease pathogenic bacteria
文献类型： 外文期刊
作者： Song, Yujie 1 ; Wang, Zeping 2 ; Liao, Jie 2 ; Zhang, Xiaoqiu 2 ; Yan, Jun 2 ; Luo, Hu 1 ; Huang, Ke-Jing 1 ; Tan, Xuecai 1 ; Ya, Yu 2 ;
作者机构： 1.Guangxi Minzu Univ, Guangxi Collaborat Innovat Ctr Chem & Engn Forest, Sch Chem & Chem Engn, Educ Dept Guangxi Zhuang Autonomous Reg,Lab Opt el, Nanning 530006, Peoples R China
2.Guangxi Acad Agr Sci, Nanning 530007, Peoples R China
关键词： Sugarcane pokkah boeng disease pathogenic; bacteria; Noble metal nanoparticles/metal oxide; nanozymes; Dual-electrode signal amplification
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2024 年 281 卷
页码：
收录情况： SCI
摘要： Sugarcane is a crop with significant economic importance worldwide. However, pokkah boeng disease poses a serious threat to its production and the sustainable development. There is a pressing necessity for precise and portable detection methods. We develop a dual-electrode signal amplification biosensing platform, for highly sensitive detection of sugarcane pokkah boeng disease pathogenic bacteria. This innovative platform integrates highly catalytic AuNPs/Mn3O4 nanozymes with N-GDY, along with a target-induced development of DNA nanostructure arrays. AuNPs/N-GDY serves as dual electrode substrates, and AuNPs/Mn3O4 nanozymes are surface-loaded as the bioanode. The biocathode is constructed by introducing DNA nanospace arrays onto the electrode through target-induced methods. [Ru(NH3)6]3+ is embedded into the nucleic acid double-helix scaffold via electrostatic adsorption, generating an EOCV signal that is strongly correlated with the target concentration. To further enhance sensitivity, the detection platform is combined with a capacitor to amplify the detection signal, utilizing its high power density, which results in a 22.5-fold increase in sensitivity. The method offers a linear detection range of 0.0001 to 10,000 pM and an detection limit of 32.5 aM (S/N = 3). This method supplies a novel approach for real-time monitoring and competent oversight of pokkah boeng disease pathogenic bacteria.
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