A simple and rapid method for isolating high-quality RNA from kenaf with high polysaccharide and polyphenol contents
文献类型： 外文期刊
作者： Liao, Xiaofang 1 ; Zhao, Yanhong 1 ; Li, Hongwei 2 ; Hou, Wenhuan 1 ; Tang, Xingfu 1 ; Zhou, Ruiyang 2 ;
作者机构： 1.Guangxi Acad Agr Sci, Cash Crop Res Inst, Nanning 530007, Peoples R China
2.Guangxi Univ, Coll Agr, Key Lab Plant Genet & Breeding, Nanning 530005, Peoples R China
3.Shaoyang Univ, Fac Mech & Energy Engn, Shaoyang 422099, Peoples R China
关键词： high polysaccharide and polyphenol contents; kenaf (Hibiscus cannabinus); northern blot analysis; RNA isolation; RT-PCR
期刊名称：BIOTECHNIQUES （ 影响因子：2.7； 五年影响因子：2.5 ）
ISSN： 0736-6205
年卷期： 2023 年 75 卷 5 期
页码：
收录情况： SCI
摘要： The extraction of high-quality RNA from kenaf is essential for genetic and molecular biology research. However, the presence of high levels of polysaccharide and polyphenol compounds in kenaf poses challenges for RNA isolation. We proposed a simplified, time-saving and cost-effective method for isolating high quantities of RNA from various kenaf tissues. This method exhibited superior efficiency in RNA isolation compared with the conventional cetyltrimethylammonium bromide method and demonstrated greater adaptability to different samples than commercial kits. Furthermore, the high-quality RNA obtained from this method was successfully utilized for RT-PCR, real-time RT-PCR and northern blot analysis. Moreover, this proposed protocol also enables the acquisition of both high-quality and -quantity gDNA through RNase A treatment. In addition, the effectiveness of this approach in isolating high-quality RNA from other plant species has been experimentally confirmed.
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