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Comparison on Characterization of Longan (Dimocarpus longan Lour.) Polyphenoloxidase Using Endogenous and Exogenous Substrates

文献类型： 外文期刊

作者： Sun, Jian 2 ; Zhang, Ezhen 1 ; Xu, Liangxiong 3 ; Li, Zhichun 1 ; Wang, Zhenxing 1 ; Li, Changbao 1 ;

作者机构： 1.Guangxi Acad Agr Sci, Inst Agrofood Sci & Technol, Nanning 530007, Peoples R China

2.Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China

3.Chinese Acad Sci, S China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China

关键词： Longan (Dimocarpus longan Lour.);polyphenoloxidase;endogenous substrate;exogenous substrate;characterization

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

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收录情况： SCI

摘要： Longan polyphenoloxidase (PPO) was extracted and partially purified using ammonium sulfate precipitation and dialysis. The PPO characterizations were compared using endogenous substrate (-)-epicatechin and exogenous substrate catechol. The optimal pH and optimal temperature for the PPO activity were different when reacting with both substrates. The addition qf ethylenediaminete-traacetic acid disodium salt into both substrate-enzyme systems exhibited the same lowest inhibition of the PPO activity. L-Ascorbic acid and L-cysteine were the best inhibitors to endogenous substrate-enzyme system, while L-ascorbic acid and glutathione were most effective inhibitors to exogenous substrate-enzyme system. Cupric (Cu~(2+)), ferric (Fe~(3+)), and ferrous (Fe~(2+)) ions accelerated the enzymatic-catalyzed reactions of both substrates. Kinetic analysis indicated that longan PPO strongly bound endogenous substrate but possessed a higher catalytic efficiency to exogenous substrate, and moreover, (-)-epicatechin was determined as the optimal substrate for longan PPO. This study is useful to exactly illuminate the enzymatic-catalyzed browning mechanism of postharvest longan fruit.

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