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Sugarcane ScDREB2B-1 Confers Drought Stress Tolerance in Transgenic Nicotiana benthamiana by Regulating the ABA Signal, ROS Level and Stress-Related Gene Expression

文献类型： 外文期刊

作者： Chen, Yufeng 1 ; Li, Zhu 1 ; Sun, Tingting 1 ; Wang, Dongjiao 1 ; Wang, Zhoutao 1 ; Zhang, Chang 1 ; Que, Youxiong 1 ; Guo, Jinlong 1 ; Xu, Liping 1 ; Su, Yachun 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Key Lab Sugarcane Biol & Genet Breeding, Minist Agr & Rural Affairs, Fuzhou 350002, Peoples R China

2.Guangxi Acad Agr Sci, Key Lab Sugarcane Biotechnol & Genet Improvement, Minist Agr & Rural Affairs, Sugarcane Res Inst, Nanning 530007, Peoples R China

3.Guangxi Acad Agr Sci, Guangxi Key Lab Sugarcane Genet Improvement, Sugarcane Res Inst, Nanning 530007, Peoples R China

关键词： sugarcane; DREB transcription factor; expression pattern analysis; self-activation activity; drought tolerance

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 17 期

页码：

收录情况： SCI

摘要： The dehydration-responsive element-binding protein (DREB) is a subgroup member of the AP2/ERF family and actively participates in the response of plants to abiotic stress. Although DREB genes have been studied in a variety of plant species, there are few reports of DREB genes in sugarcane (Saccharum spp.). In this study, a novel full-length cDNA sequence of the ScDREB2B-1 gene was cloned from the Saccharum hybrid ROC22, whose encoding protein contained only one AP2-conserved domain and was clustered into the DREB (A-2) subgroup. The diverse promoter elements in the ScDREB2B-1 gene and the accumulated transcripts of its homologous gene (SsAP2/ERF-107) in S. spontaneum under drought stress suggest that the ScDREB2B-1 gene may play a role in drought response. In addition, reverse transcription quantitative PCR analysis showed that the expression level of the ScDREB2B-1 gene was upregulated in the root and leaf of ROC22 under polyethylene glycol, sodium chloride and abscisic acid (ABA) treatments. The yeast two-hybrid experiment demonstrated that ScDREB2B-1 had transcriptional self-activation activity. Compared with wild-type plants, the overexpression of the ScDREB2B-1 gene improved the drought tolerance of the transgenic Nicotiana benthamiana by activating the ABA pathway to enhance the expression of the ABA-responsive gene (NbNCED) and ABA content, regulate the intracellular reactive oxygen species (ROS) level (enhance the transcripts of ROS synthase-related gene NbRbohB and the activities of catalase, peroxidase and superoxide dismutase) and increase the relative water content, proline content and expression level of osmotic stress-related genes (NbERD and NbLEA). Collectively, our data indicate that ScDREB2B-1 is a stress-inducible and ABA-responsive transcription factor gene that responds to drought stress by regulating ABA signaling, ROS levels and stress-related gene expression. This study contributes to a better understanding of the biological function of ScDREB2B-1, which could serve as a foundation for future resistance breeding in sugarcane.

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