文献类型： 外文期刊

作者： Li, Z. ¹ ; Chang, S. ¹ ; Lin, L. ² ; Li, Y. ² ; An, Q. ¹ ;

作者机构： 1.Zhejiang Univ, Inst Biotechnol, Minist Agr, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou 310058, Zhejiang, Peoples R China

2.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China

3.Guangxi Acad Agr Sci, Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning, Peoples R China

关键词： nitrogen: 7727-37-9;ninhydrin: 485-47-2;1-aminocyclopropane-1-carboxylate deaminase: 69553-48-6;EC 3.5.99.7;1-aminocyclopropane-1-carboxylate: 22059-21-8;enzyme activity

期刊名称：LETTERS IN APPLIED MICROBIOLOGY （ 影响因子：2.858； 五年影响因子：2.776 ）

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收录情况： SCI

摘要： Aims: 1-Aminocyclopropane-1-carboxylate (ACC) deaminase activity is an efficient marker for bacteria to promote plant growth by lowering ethylene levels in plants. We aim to develop a method for rapidly screening bacteria containing ACC deaminase, based on a colorimetric ninhydrin assay of ACC.Methods and Results: A reliable colorimetric ninhydrin assay was developed to quantify ACC using heat-resistant polypropylene chimney-top 96-well PCR plates, having the wells evenly heated in boiling water, preventing accidental contamination from boiling water and limiting evaporation. With this method to measure bacterial consumption of ACC, 44 ACC-utilizing bacterial isolates were rapidly screened out from 311 bacterial isolates that were able to grow on minimal media containing ACC as the sole nitrogen source. The 44 ACC-utilizing bacterial isolates showed ACC deaminase activities and belonged to the genus Burkholderia, Pseudomonas or Herbaspirillum.Conclusions: Determination of bacterial ACC consumption by the PCR-plate ninhydrin-ACC assay is a rapid and efficient method for screening bacteria containing ACC deaminase from a large number of bacterial isolates.Significance and Impact of the Study: The PCR-plate ninhydrin-ACC assay extends the utility of the ninhydrin reaction and enables a rapid screening of bacteria containing ACC deaminase from large numbers of bacterial isolates.