您好，欢迎访问广西农业科学院 机构知识库！

Map-based cloning and characterization of BPH29, a B3 domain-containing recessive gene conferring brown planthopper resistance in rice

文献类型： 外文期刊

作者： Wang, Ying 1 ; Cao, Liming 2 ; Zhang, Yuexiong 3 ; Cao, Changxiang 1 ; Liu, Fang 3 ; Huang, Fengkuan 5 ; Qiu, Yongfu 3 ;

作者机构： 1.Fudan Univ, Sch Life Sci, Inst Genet, State Key Lab Genet Engn, Shanghai 200438, Peoples R China

2.Shanghai Acad Agr Sci, Crop Breeding & Cultivat Res Inst, Shanghai 201403, Peoples R China

3.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530004, Peoples R China

4.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China

5.Guangxi Acad Agr S

关键词： B3 domain protein;BPH29;brown planthopper resistance;map-based cloning;Oryza sativa L;plant-insect defence;recessive gene

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.992； 五年影响因子：7.86 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A brown planthopper resistance recessive gene, BPH29, was cloned which contained a B3 DNA-binding domain and conferred resistance by a mechanism that was similar to plant defence against pathogens.Rice (Oryza sativa L.) production, essential for global food security, is threatened by the brown planthopper (BPH). The breeding of host-resistant crops is an economical and environmentally friendly strategy for pest control, but few resistance gene resources have thus far been cloned. An indica rice introgression line RBPH54, derived from wild rice Oryza rufipogon, has been identified with sustainable resistance to BPH, which is governed by recessive alleles at two loci. In this study, a map-based cloning approach was used to fine-map one resistance gene locus to a 24kb region on the short arm of chromosome 6. Through genetic analysis and transgenic experiments, BPH29, a resistance gene containing a B3 DNA-binding domain, was cloned. The tissue specificity of BPH29 is restricted to vascular tissue, the location of BPH attack. In response to BPH infestation, RBPH54 activates the salicylic acid signalling pathway and suppresses the jasmonic acid/ethylene-dependent pathway, similar to plant defence responses to biotrophic pathogens. The cloning and characterization of BPH29 provides insights into molecular mechanisms of plant-insect interactions and should facilitate the breeding of rice host-resistant varieties.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广西农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台