文献类型： 外文期刊

作者： Sun, Hua-peng ¹ ; Li, Fang ¹ ; Cao, Xiong-jun ¹ ; Ruan, Qin-mei ¹ ; Zhong, Xiao-hong ¹ ;

作者机构： 1.Hunan Agr Univ, Hort & Landscape Coll, Changsha 410128, Hunan, Peoples R China

2.Natl Ctr Citrus Improvement, Changsha 410128, Hunan, Peoples R China

3.Guangxi Acad Agr Sci, Grape & Wine Res Inst, Nanning 530007, Guangxi, Peoples R China

关键词： Hedera plant;SRAP molecular marker;Cluster analysis;HPLC detection;Medicinal value

期刊名称：BIOCHEMICAL SYSTEMATICS AND ECOLOGY （ 影响因子：1.381； 五年影响因子：1.351 ）

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收录情况： SCI

摘要： Hedera plants are ornamental vines which are regarded as traditional medicinal plants. These plants are widely cultivated worldwide; moreover, new species and varieties have been discovered. For the first time, molecular markers based on sequence-related amplified polymorphism (SRAP) were used in this study to classify species and to analyse the genetic relationship of 21 Hedera plant varieties which are commonly cultivated in China. We also employed high-performance liquid chromatography (HPLC) to evaluate the medicinal value of these plants by measuring their hederacoside C contents. Results of SRAP -PCR and cluster analyses show that the SRAP molecular markers can successfully be applied in Hedera plants and are available. In addition, HPLC detection results indicate that the Chinese wild species in group I contain less than 30 mg/g hederacoside C and do not comply with the European Pharmacopoeia standards. By contrast, groups II and III exhibit high medicinal value and accord with these standards. (C) 2015 Elsevier Ltd. All rights reserved.