您好，欢迎访问广西农业科学院 机构知识库！

A Comparative Analysis of the atp8 Gene Between a Cytoplasmic Male Sterile Line and Its Maintainer and Further Development of a Molecular Marker Specific to Male Sterile Cytoplasm in Kenaf (Hibiscus cannabinus L.)

文献类型： 外文期刊

作者： Liao, Xiaofang 2 ; Zhao, Yanhong; Chen, Peng 1 ; Zhou, Bujin 1 ; Diao, Yong 1 ; Yu, Mingli 1 ; Huang, Zhipeng 1 ; Zhou 1 ;

作者机构： 1.Guangxi Univ, Key Lab Plant Genet Breeding, Nanning 530005, Guangxi, Peoples R China

2.Guangxi Univ, Coll Agr, Nanning 530005, Guangxi, Peoples R China

3.Guangxi Univ, Coll Agr, Na

关键词： Kenaf(Hibiscus cannabinus L.);CMS line and its maintainer line;atp8;Molecular marker

期刊名称：PLANT MOLECULAR BIOLOGY REPORTER （ 影响因子：1.595； 五年影响因子：2.042 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, the atp8 gene was cloned from the cytoplasmic male sterile (CMS) line UG93A and its maintainer line UG93B in kenaf. Its DNA sequence analysis showed that atp8 containing 480-bp, encoding 159 amino acid residues, and a 9-bp insertion was found at the 3'flanking sequence in UG93A compared with UG93B. The cDNA sequence of atp8 analyzed by RT-PCR indicated that there were five loci edited, but six loci edited in UG93B. The editing frequencies were higher in sterile cytoplasm than in fertile cytoplasm. The relative expression of atp8 analyzed by real-time PCR showed that the expressed level of atp8 in UG93A was lower than that of its maitainer UG93B and its F-1 hybrid UG93A/992 (a restore line). Furthermore, based on the difference of the 9-bp differences at the 3'flanking sequence of atp8 between UG93A and UG93B, a molecular marker specific to male sterile cytoplasm was developed, which can be used for indentifying whether any germplasm of kenaf is male sterile cytoplasm or male fertile cytoplasm.

  • 相关文献

[1]Verification of Progeny from Crosses Between Sugarcane (Saccharum spp.) and an Intergeneric Hybrid (Erianthus arundinaceus x Saccharum spontaneum) with Molecular Makers. Liu, Xi-Hui,Zhang, Rong-Hua,Zhou, Hui,Liao, Jiang-Xiong,Duan, Wei-Xing,Zhang, Ge-Min,Gao, Yi-Jing,Liu, Xi-Hui,Zhang, Rong-Hua,Zhou, Hui,Liao, Jiang-Xiong,Duan, Wei-Xing,Zhang, Ge-Min,Gao, Yi-Jing,Liu, Xi-Hui,Zhang, Rong-Hua,Zhou, Hui,Liao, Jiang-Xiong,Duan, Wei-Xing,Zhang, Ge-Min. 2015

[2]A comparative study of the atp9 gene between a cytoplasmic male sterile line and its maintainer line and further development of a molecular marker specific for male sterile cytoplasm in kenaf (Hibiscus cannabinus L.). Chen, Peng,Liao, Xiaofang,Zhou, Bujin,Liao, Jian,Huang, Zhipeng,Kong, Xiangjun,Zhou, Ruiyang,Zhao, Yanhong.

[3]Mutation in the coding sequence of atp6 are associated with male sterile cytoplasm in kenaf (Hibiscus cannabinus L.). Liao, Xiaofang,Zhou, Bujin,Zhou, Yaoyao,Zhou, Ruiyang,Zhao, Hongtao,Zhou, Yaoyao.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广西农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台