文献类型： 外文期刊

作者： Tan, Seok-Tyug ¹ ; Ismail, Amin ² ; Hamid, Muhajir ³ ; Chong, Pei-Pei ⁴ ; Sun, Jian ⁵ ; Tan, Seok-Shin ⁷ ;

作者机构： 1.Management & Sci Univ, Fac Hlth & Life Sci, Dept Healthcare Profess, Shah Alam, Selangor, Malaysia

2.Univ Putra Malaysia, Fac Med & Hlth Sci, Dept Nutr & Dietet, Upm Serdang, Malaysia

3.Univ Putra Malaysia, Fac Biotechnol & Biomol Sci, Dept Microbiol, Upm Serdang, Malaysia

4.Taylors Univ, Fac Hlth & Med Sci, Sch Biosci, Subang Jaya, Malaysia

5.Inst Agrofood Sci & Technol, Guangxi, Peoples R China

6.Guangxi Acad Agr Sci, Guangxi, Peoples R China

7.Int Med Univ, Sch Hlth Sci, Dept Nutr & Dietet, Bukit Jalil, Malaysia

关键词： Adipogenesis; BRIN BD-11; hypoglycemic; 3T3-L1 adipocytes; Soya bean husk

期刊名称：NUTRITION & FOOD SCIENCE （ 影响因子：1.2； 五年影响因子：1.5 ）

ISSN： 0034-6659

年卷期： 2019 年 49 卷 6 期

页码：

收录情况： SCI

摘要： Purpose Literature has shown that phenolic acids and flavonoids are bearing with hypoglycemic and anti-adipogenic properties. Therefore, this study aims to evaluate the possibility of phenolic-rich soya bean husk powder extract (SHPE) in combating diabetes and obesity using in vitro models. Design/methodology/approach The hypoglycemic properties were evaluated by determining the ability of SHPE (25-100 mu g/mL) in inhibiting a-amylase and a-glucosidase enzymes and in triggering insulin secretion in BRIN-BD11 cells. Murine 3T3-L1 adipocytes were used for evaluating the anti-adipogenic properties of SHPE through the determination of relative lipid accumulation, triglyceride content and glycerol-3-phosphate dehydrogenase (GPDH) activity. Findings The hypoglycemic properties of SHPE was in the dose-dependent manner, where 100 mu g SHPE/mL exhibited a significant higher (p < 0.05) a-amylase inhibitory activity (56.8 +/- 0.11 per cent) and insulin secretion activity (0.73 +/- 0.02 mu g/l) against other concentrations. In contrast to the aforementioned findings, a significant lower a-glucosidase inhibitory activity (52.0 +/- 0.44 per cent) was also observed in 100 mu g SHPE/mL. Nevertheless, findings revealed that all the SHPE were able to inhibit the activity of a-amylase and a-glucosidase and stimulated the insulin secretion in BRIN-BD11 cells. On the other hand, the anti-adipogenic properties of SHPE were in the reverse dose-dependent manner, where 100 mu g SHPE/mL demonstrated a significant lower (p < 0.05) relative lipid accumulation (48.5 +/- 0.03 per cent), intracellular triglyceride content (5.7 +/- 0.07 mg/dL) and GPDH activity (1.0 +/- 0.01 mU/mL). These findings reflected that 100 mu g SHPE/mL was a potent anti-adipogenic agent when compared with other concentrations. In conclusion, soya husk could emerge as a potential hypoglycemic and anti-adipogenic agents in in vitro models. Originality/value This was the first study to explore the effectiveness of phytochemicals derived from soya bean husk in ameliorating hyperglycemia and adipogenesis. Promising findings that derived from the present study could enable the scientists to re-evaluate the potential use of agricultural wastes, especially in the formulation of nutraceuticals.