文献类型： 外文期刊

作者： Yang, Shiying ¹ ; Pang, Wen ¹ ; Ash, Gavin ¹ ; Harper, John ¹ ; Carling, Jason ² ; Wenzl, Peter; Huttner, Eric; Zon ¹ ;

作者机构： 1.DAtT PL, Canberra, ACT 2600, Australia; Guangxi Acad Agr Sci, Guangxi 530007, Peoples R China; Charles Sturt Univ, Sch Agr & Vet Sci, EH Graham Ctr Agr Innovat, Wagga Wagga, NSW 2678, Australia; Ctr Appl Mol Biol Int Agr, Canberra, ACT 2601, Australia; Chinese Acad Agr Sci, Beijing 100081, Peoples R China

2.DAtT PL, Canberra, ACT 2600, Australia; Guangxi Acad Agr Sci

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2006 年 113 卷 4 期

页码：

收录情况： SCI

摘要： Understanding the distribution of genetic diversity among individuals, populations and gene pools is crucial for the efficient management of germplasm collections and breeding programs. Diversity analysis is routinely carried out using sequencing of selected gene(s) or molecular marker technologies. Here we report on the development of Diversity Arrays Technology (DArT) for pigeonpea (Cajanus cajan) and its wild relatives. DArT tests thousands of genomic loci for polymorphism and provides the binary scores for hundreds of markers in a single hybridization-based assay. We tested eight complexity reduction methods using various combinations of restriction enzymes and selected PstI/HaeIII genomic representation with the largest frequency of polymorphic clones (19.8%) to produce genotyping arrays. The performance of the PstI/HaeIII array was evaluated by typing 96 accessions representing nearly 20 species of Cajanus. A total of nearly 700 markers were identified with the average call rate of 96.0% and the scoring reproducibility of 99.7%. DArT markers revealed genetic relationships among the accessions consistent with the available information and systematic classification. Most of the diversity was among the wild relatives of pigeonpea or between the wild species and the cultivated C. cajan. Only 64 markers were polymorphic among the cultivated accessions. Such narrow genetic base is likely to represent a serious impediment to breeding progress in pigeonpea. Our study shows that DArT can be effectively applied in molecular systematics and biodiversity studies.