Exploring the dual roles of sec-dependent effectors from Candidatus Liberibacter asiaticus in immunity of citrus plants
文献类型： 外文期刊
作者： Li, Xue 1 ; Zhou, Yue 1 ; Chen, Hang 1 ; Guo, Zetian 1 ; Zhang, Jinlian 3 ; Chen, Wenli 1 ;
作者机构： 1.South China Normal Univ, Coll Biophoton, Sch Optoelect Sci & Engn, MOE Key Lab Laser Life Sci, Guangzhou 510631, Peoples R China
2.South China Normal Univ, Inst Laser Life Sci, Coll Biophoton,Guangzhou Key Lab Spectral Anal & F, Sch Optoelect Sci & Engn,Guangdong Prov Key Lab La, Guangzhou 510631, Peoples R China
3.Guangxi Acad Agr Sci, Microbiol Res Inst, Nanning 530007, Peoples R China
关键词：
Citrus huanglongbing;
期刊名称：PLANT CELL REPORTS （ 影响因子：4.5； 五年影响因子：6.1 ）
ISSN： 0721-7714
年卷期： 2025 年 44 卷 2 期
页码：
收录情况： SCI
摘要： Key messageThe three SDEs of CLas were expressed in citrus leaves by AuNPs-PEI mediated transient expression system, and promoted the proliferation of CLas and inhibited citrus immunity.AbstractHuanglongbing (HLB) is the most severe bacterial disease of citrus caused by Candidatus Liberibacter asiaticus (CLas). CLas suppress host immune responses and promote infection by sec-dependent effectors (SDEs), thus insight into HLB pathogenesis is urgently needed to develop effective management strategies. In this study, we focused on the roles of SDE4310, SDE4435 and SDE4955 in citrus. We found that the expression of SDE4310, SDE4435 and SDE4955 to increase with increasing citrus immune genes CsPR1, CsPR2, CsPR5, CsNPR1, CsRBOHD, CsMAP3K and CsBIK1, suggesting that the level of citrus immunity could be judged by the expression of SDE. To further explore the relationship between these three SDEs and citrus immunity, we established a transient expression system in citrus leaves, using gold nanoparticle-polyethyleneimine (AuNPs-PEI) to deliver recombinant plasmid containing SDE4310, SDE4435 or SDE4955 respectively into citrus leaves. Results showed that SDE4310, SDE4435 and SDE4955 were successfully expressed in citrus leaves using this transient expression system, and found that SDE4310, SDE4435 and SDE4955 could promote the CLas proliferation by decreasing the immune gene expression of the citrus. Additionally, we used AuNPs-PEI to deliver siRNA4310 to citrus cells, significantly reducing the expression of SDE4310 within 3 days. Although the suppression of SDE4310 expression did not inhibit the CLas proliferation, it increased the expression level of CsPR1, CsNPR1 and CsBIK1. This is also the first time that AuNPs-PEI has been found to be able to deliver exogenous plasmids into citrus cells and express the target protein, providing a new method for future studies on citrus HLB.
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