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Fine Mapping and Functional Analysis of Major Regulatory Genes of Soluble Solids Content in Wax Gourd (Benincasa hispida)

文献类型： 外文期刊

作者： Wu, Wenting 1 ; Wang, Peng 2 ; Huang, Xiaochun 1 ; Su, Liwen 1 ; Lv, Haixuan 1 ; Gou, Jiquan 1 ; Cheng, Zhikui 1 ; Ma, Lianlian 1 ; Yu, Wenjin 1 ; Liu, Zhengguo 1 ;

作者机构： 1.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China

2.Guangxi Acad Agr Sci, Inst Vegetable Res, Nanning 530004, Peoples R China

关键词： wax gourd; soluble solids content; BSA; QTL; NADP-dependent malic enzyme

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 13 期

页码：

收录情况： SCI

摘要： Soluble solids content (SSC) is an important quality trait of wax gourd, but reports about its regulatory genes are scarce. In this study, the SSC regulatory gene BhSSC2.1 in wax gourd was mined via quantitative trait locus (QTL) mapping based on high-density genetic mapping containing 12 linkage groups (LG) and bulked segregant analysis (BSA)-seq. QTL mapping and BSA-seq revealed for the first time that the SSC QTL (107.658-108.176 cM) of wax gourd was on Chr2 (LG2). The interpretable phenotypic variation rate and maximum LOD were 16.033% and 6.454, respectively. The QTL interval contained 13 genes. Real-time fluorescence quantitative expression analysis, functional annotation, and sequence analysis suggested that Bch02G016960, named BhSSC2.1, was a candidate regulatory gene of the SSC in wax gourd. Functional annotation of this gene showed that it codes for a NADP-dependent malic enzyme. According to BhSSC2.1 sequence variation, an InDel marker was developed for molecular marker-assisted breeding of wax gourd. This study will lay the foundation for future studies regarding breeding and understanding genetic mechanisms of wax gourd.

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