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Cloning and functional analysis of 1-deoxy-D-xylulose-5-phosphate synthase (DXS) in Santalum album L

文献类型： 外文期刊

作者： Chen, Xiaohong 1 ; Zhang, Yueya 1 ; Yan, Haifeng 3 ; Niu, Meiyun 1 ; Xiong, Yuping 1 ; Zhang, Xinhua 1 ; Li, Yuan 1 ; da Silva, Jaime A. Teixeira 4 ; Ma, Guohua 1 ;

作者机构： 1.Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou 510650, Peoples R China

2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China

3.Guangxi Acad Agr Sci, Nanning 530007, Peoples R China

4.Ikenobe 3011-2, Kagawa 7610799, Japan

关键词： Chlorophylls and carotenoids; Ethylene; Jasmonate; MEP pathway; SaDXS1A; 1B; Sandalwood; Sesquiterpenes

期刊名称：GENE （ 影响因子：3.5； 五年影响因子：3.3 ）

ISSN： 0378-1119

年卷期： 2023 年 851 卷

页码：

收录情况： SCI

摘要： The commercial value of Santalum album L. lies in its aromatic heartwood and essential oil. Sesquiterpenes are the main components of sandal essential oil, and these are synthesized through the plant's mevalonate (MVA) and methylerythritol phosphate (MEP) pathways. In this study, the first key rate-limiting enzyme, 1-deoxy-D- xylulose-5-phosphate synthase (SaDXS), was investigated to provide a theoretical molecular basis for the san-dalwood MEP sesquiterpene biosynthetic pathway. The biofunctions of SaDXS were also analyzed. SaDXS pro-moters were successfully cloned from a seven-year-old S. album tree. SaDXS1A/1B promoter activity was verified by a beta-glucuronidase (GUS) assay and by analyzing cis-acting elements of the promoters, which carried light-and methyl jasmonate (MeJA)-responsive signals. In an experiment involving yellow S. album seedlings, exposure to light upregulated SaDXS1A/1B expression and increased chlorophyll and carotenoid contents when overex-pressed in Arabidopsis thaliana. Analysis of the expression of SaDXS1A/1B and SaSSy, key genes of santalol biosynthesis, revealed SaDXS1A expression in all tissues whereas SaDXS1B was expressed in tissues that con-tained photosynthetic pigments, such as stems, leaves and flowers. Sandal seedlings exogenously treated with two hormones, MeJA and ethylene, revealed similar expression patterns for SaDXS1A/1B and SaSSy. Sandal seedlings were treated with an inhibitor of DXS, clomazone, but showed no significant changes in the contents of alpha-santalene, beta-santalene and alpha-santalol between treatment and control groups. These results suggest that SaDXS1A/1B play a role in the synthesis of sandalwood sesquiterpenes, providing carbon for downstream sec-ondary metabolites. SaDXS1A/1B also play a role in the biosynthesis of chlorophyll, carotenoids, and primary metabolites.

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