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Methods for Estimation of Nitrogen Components in Plants and Microorganisms

文献类型： 外文期刊

作者： Singh, Pratiksha 1 ; Singh, Rajesh Kumar 1 ; Song, Qi-Qi 1 ; Li, Hai-Bi 1 ; Yang, Li-Tao 1 ; Li, Yang-Rui 1 ;

作者机构： 1.Guangxi Acad Agr Sci, Chinese Acad Agr Sci, Key Lab Sugarcane Biotechnol & Genet Improvement, Guangxi Key Lab Sugarcane Genet Improvement,Minis, Nanning, Guangxi, Peoples R China

2.Guangxi Univ, Coll Agr, Nanning, Guangxi, Peoples R China

3.Guangxi Key Lab Crop Genet Improvement & Biotechn, Nanning, Guangxi, Peoples R China

关键词： Acetylene reduction assay; Combustion method; Kjeldahl; Microbial bioassay; N-15(2)

期刊名称：NITROGEN METABOLISM IN PLANTS: METHODS AND PROTOCOLS

ISSN： 1064-3745

年卷期： 2020 年 2057 卷

页码：

收录情况： SCI

摘要： Nitrogen (N-2) is the most necessary element in the atmosphere, it is an energetic micronutrient for plant growth and development after water, besides its key role in chlorophyll production, which is crucial for photosynthesis process. Biological nitrogen fixation is measured to be the most potent method to deliver a fixed way of nitrogen to the plants. Plant depends on free-living and symbiotic microbes present in the soil for nitrogen because it cannot be absorbed by the plant itself directly from the atmosphere. Many techniques were reported in the laboratory for nitrogen estimation till now, but Kjeldahl digestion and acetylene reduction assay (ARA) techniques became the most popular. In this chapter, we focus on the most common and popular methods used to determine plant N-2; awareness obtained through the wide application of these methods should offer the source for the N-2 fixation rate in agriculture system.

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