Multiple signal amplification strategy induced by biomarkers of lung cancer: A self-powered biosensing platform adapted for smartphones
文献类型： 外文期刊
作者： Song, Yujie 1 ; Ya, Yu 2 ; Cen, Xiaotian 1 ; Tang, Danyao 1 ; Shi, Jinyue 1 ; Wu, Yeyu 1 ; Luo, Hu 1 ; Huang, Ke- Jing 1 ; Tan, Xuecai 1 ; Yan, Feiyan 1 ;
作者机构： 1.Guangxi Minzu Univ, Sch Chem & Chem Engn, Key Lab Chem & Engn Forest Prod, State Ethn Affairs Commiss,Educ Dept Guangxi Zhuan, Nanning 530006, Peoples R China
2.Guangxi Acad Agr Sci, Inst Agr Prod Qual Safety & Testing Technol, Nanning 530007, Peoples R China
关键词： Capacitor-enhanced enzyme biofuel cell; DNAzyme-mediated dual-strand displacement; amplification strategy; Smartphone-assisted real-time detection
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2024 年 264 卷
页码：
收录情况： SCI
摘要： Lung cancer is a major malignant cancer with low survival rates, and early diagnosis is crucial for effective treatment. Herein, a biosensing platform that is self-powered derived from a capacitor-coupled EBFC has been developed for ultra-sensitive real-time identification of microRNA-21 (miRNA-21) with the assistance of a mobile phone. The flexible substrate of the platform is prepared on a carbon paper modified with graphdiyne and gold nanoparticles. The biosensor employs DNAzyme-mediated dual strand displacement amplification, which enhances the signal output intensity of the EBFC and improves selectivity. The coupling of the capacitor with the EBFC significantly amplifies the sensing signal, causing a 10.6-fold surge in current respond and further improving the sensitivity of the sensing platform. The established detection approach demonstrates a linear relationship varied from 0.0001 to 10,000 pM, with a sensitivity down to 32.3 aM as the minimum detectable limit, which has been effectively utilized for detecting miRNA-21 in practical samples. This sensing system provides strong support for the construction of portable detection devices, and the strategy of the platform construction provides an effective method for ultra-sensitive and accurate detection of miRNA, holding great potential in clinical diagnosis, prognosis evaluation, and drug screening for cancer.
- 相关文献
作者其他论文 更多>>
-
A dual-mode strategy for early detection of sugarcane pokkah boeng disease pathogen: A portable sensing device based on Cross-N DNA framework and MoS2@GDY
作者：Song, Yujie;Wu, Qingnian;Su, Jing;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Wang, Zeping;Liao, Jie;Zhang, Xiaoqiu;Ya, Yu
关键词：Portable dual-mode detection device; Exo III exonuclease-assisted CHA strategy; Cross-N DNA framework; Sugarcane pokkah boeng disease pathogen; Smartphone-assisted analysis
-
An intelligent sensing platform for sugarcane pokkah boeng disease based on CHA-driven RCA cascade DNAzyme amplification technology and clustered DNA nanoflowers
作者：Wu, Qingnian;Song, Yujie;Jin, Chenchen;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Ya, Yu;Wang, Zeping;Liao, Jie;Yan, Feiyan
关键词：Sugarcane pokkah boeng disease; Cascade amplification technology; Bundle DNA nanoflowers; Smartphone assisted detection
-
Sensitivity-enhanced self-powered biosensing platform for detection of sugarcane smut using Mn-doped ZIF-67, RCA-DNA nano-grid array and capacitor
作者：Fu, Bingtao;Che, Rongshuai;Wu, Yeyu;Luo, Hu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Yan, Feiyan;Ya, Yu
关键词：Sugarcane smut; Self-powered platform; DNA nano-grid array; Cascade signal amplification
-
From leaf to lab-on-cloth: Spatial DNA nanorobotics and 2D graphyne synergy enable ultra-precise electrochemical tracking of sugarcane pokkah boeng disease
作者：Wu, Qingnian;Jin, Chenchen;Zhao, Yinxia;Feng, Defen;Huang, Ke-Jing;Tan, Xuecai;Ya, Yu;Yan, Feiyan;Xie, Shengyu
关键词：Sugarcane pokkah boeng disease; S-GDY; Dual strand displacement amplification; Dual-signal readout; Spatial DNA nanorobotics
-
High-Performance Biosensing Platforms Based on Enzyme-Linked Nucleic Acid Amplification Regulated by Synergistic Allosteric Hairpin Catalysis of Bimetallic Nanozymes and Its Mechanisms
作者：Ma, Yunzhi;Jin, Chenchen;Wu, Yeyu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Yan, Feiyan;Ya, Yu
关键词：
-
Super signal-enhancement biosensing platform for precise target recognition based on rolling circle-hybridization chain dual linear cascade amplification technology
作者：Tang, Danyao;Xiong, Chunyuan;Wu, Yeyu;Luo, Hu;Yan, Jun;Huang, Ke-Jing;Tan, Xuecai;Ya, Yu
关键词：Double-layer linear cascade amplification technology; Precise target identification; Super signal amplification; Efficient conversion
-
Highly Sensitive Detection of Water in Organic Solvents Using Pyrene-Phenol-based Fluorescent Probe
作者：Liang, Jing;Wang, Yan-Li;Jiang, Cui-Wen;Huang, Xiao-Chuan;Tang, Li;Li, Tao;Ya, Yu;Ning, De-Jiao;Xie, Li-Ping;Yan, Fei-Yan
关键词：Fluorescent probe; Pyrene-phenol; Differentiation of solvents; Detection of water content
