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High sensitive and selective electrochemical aptasensor for patulin detection based on a novel assembling strategy using hierarchically porous carbon

文献类型: 外文期刊

作者: Jiang, Cuiwen 1 ; Wang, Yanli 1 ; Liang, Jing 1 ; Xie, Liping 1 ; Liang, Zhongdan 1 ; Huang, Kejing 2 ; Li, Huiling 1 ; Li, Tao 1 ; Ning, Dejiao 1 ; Tang, Li 1 ; Huang, Xiaochuan 1 ; Luo, Lihong 1 ; Ya, Yu 1 ; Yan, Feiyan 1 ;

作者机构: 1.Guangxi Acad Agr Sci, Inst Agr Prod Qual Safety & Testing Technol, Nanning 530007, Peoples R China

2.Guangxi Univ Nationalities, Sch Chem & Chem Engn, Nanning 530008, Peoples R China

关键词: Patulin; Hierarchically porous carbon; Aptasensor; Electroanalysis; Voltammetry

期刊名称:JOURNAL OF FOOD COMPOSITION AND ANALYSIS ( 影响因子:4.3; 五年影响因子:4.6 )

ISSN: 0889-1575

年卷期: 2024 年 131 卷

页码:

收录情况: SCI

摘要: Patulin (PAT) in food is a menace to human health. Hence, a sensitive and precise approach to PAT detection is significant. In this research, an innovative electrochemical assay was developed for sensitively determining PAT by assembling aptamers on a carboxylation hierarchically porous carbon (HPC-COOH) modified glassy carbon electrode. Diverse analytical methods were used to describe electrode morphology, structure, chemical composition, and electrochemistry properties. The HPC-COOH, with a large surface area, played a crucial role as a unique substrate to effectively immobilize aptamers and enhance target PATs ' recognition and capture ability. Simultaneously, the excellent electrical conductivity of HPC-COOH was significant for the signal amplification of sensors. Under optimized conditions, the aptasensor enabled efficient PAT detection in a broad concentration range (0.5 - 5.0 x10 6 ng/L) with a low detection limit (0.25 ng/L). The designed aptasensor showed high sensitivity, selectivity, reproducibility, and stability under optimized conditions. The sensors ' feasibility for practical applications was further demonstrated by analyzing apple juice and haw juice samples.

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