文献类型： 外文期刊
作者： Wei, Ping 1 ; Tang, Yayuan 1 ; Zhou, Kui 1 ; Wei, Zhen 1 ; Liu, Guoming 2 ;
作者机构： 1.Guangxi Acad Agr Sci, Agrofood Sci & Technol Res Inst, 174 East Daxue Rd, Nanning 530007, Peoples R China
2.Guangxi Key Lab Fruits & Vegetables Storage Proc T, 174 East Daxue Rd, Nanning 530007, Peoples R China
3.Guangxi Acad Agr Sci, 174 East Daxue Rd, Nanning 530007, Peoples R China
关键词： industrial hemp kernel; polysaccharide; antioxidant activity; structural identification
期刊名称：FOODS （ 影响因子：5.1； 五年影响因子：5.6 ）
ISSN：
年卷期： 2024 年 13 卷 21 期
页码：
收录情况： SCI
摘要： Polysaccharides from hemp seeds exhibit antioxidant activities in vitro and in vivo. However, crude polysaccharide quality is often low owing to the presence of fibres and pigment impurities, which are difficult to eliminate in the hemp seed shell. In this study, crude polysaccharides from hemp kernels (HKP) were obtained by hot water extraction and separated by membrane ultrafiltration into eight fractions with different molecular weights. Total antioxidant capacity and free radical scavenging (DPPH) assays were performed to evaluate the antioxidant activities of HKP and the fractions in vitro. The structural characteristics of HKP were determined using various analytical techniques. The Fe3+-reducing power of HKP was 7.65 +/- 0.22 mu mol/g, and HKP possessed the highest DPPH radical-scavenging rates (94.30 +/- 2.27%), similar to 5 mg/mL Vitamin C (Vc), which had a rate of 95%. The HKP was an acidic polysaccharide with a low molecular weight (4.21 +/- 0.12 kDa). The monosaccharide composition indicated that HKP primarily comprised mannose, ribose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose, and fucose in a molar ratio of 0.96:1.95:8.27:0.98:9.46:1.69:6.10:2.82. The molar mass of HKP was distributed widely in a triple helical conformation. This study provides a scientific basis for further research on the use of hemp polysaccharides.
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