文献类型： 外文期刊

作者： Zhang Yuexiong ¹ ; Qin Gang ³ ; Ma Qianqian ¹ ; Wei Minyi ³ ; Yang Xinghai ³ ; Ma Zengfeng ³ ; Liang Haifu ³ ; Liu Chi ³ ; L ¹ ;

作者机构： 1.Guangxi Univ, Coll Agr, Nanning 530007, Peoples R China

2.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530007, Peoples R China

3.Guangxi Acad Agr Sci, Rice Res Inst, Nanning 530007, Peoples R China

关键词： rice; brown planthopper; Bph35; bulked segregant analysis (BSA)-seq method; gene mapping

期刊名称：RICE SCIENCE （ 影响因子：3.333； 五年影响因子：4.226 ）

ISSN： 1672-6308

年卷期： 2020 年 27 卷 3 期

页码：

收录情况： SCI

摘要： An introgression line RBPH660, derived from wild rice Oryza rufipogon, showed stable resistance to brown planthopper (BPH). Segregation analysis indicated BPH resistance of RBPH660 was controlled by multiple genes/QTLs. By using the bulked segregant analysis (BSA)-seq method, two genomic regions harboring QTLs resistance to BPH were identified from 1.20 to 16.70 Mb on chromosome 4 and from 10.20 to 12.60 Mb on chromosome 9 in RBPH660, respectively. A major resistance locus, designated as Bph35 accounting for 51.27% of the phenotypic variation with a LOD score of 42.51, was mapped to the candidate region of chromosome 4 between InDel (insertion-deletion) markers PSM16 and R4M13. For fine mapping of Bph35, one simple sequence repeat and three newly developed InDel markers were used to screen the recombinants. Finally, the Bph35 locus was delimited in the region from 6.28 to 6.93 Mb and there were 18 predicted protein-encoding genes with a total of 114 non-synonymous single nucleotide polymorphism (SNP) variant sites between the resistant and susceptible parents. Out of these genes, Os04g0193950, encoding a putative NB-ARC (nucleotide-binding adaptor shared by APAF-1, R proteins and CED-4) and LRR (leucine-rich repeat) domain protein with nine non-synonymous SNP substitutions in its coding sequence regions, might be the candidate gene for Bph35. These findings would facilitate the map-based cloning of the Bph35 gene and development of resistant varieties against BPH in rice.