文献类型： 外文期刊

作者： Hua, Jinfeng ¹ ; Pan, Chao ¹ ; Huang, Yongmei ² ; Li, Yanqing ² ; Li, Huifeng ² ; Wu, Cuirong ² ; Chen, Tianyuan ² ; Ma, ¹ ;

作者机构： 1.Jiangsu Normal Univ, Inst Integrat Plant Biol, Jiangsu Key Lab Phylogen & Comparat Genom, Sch Life Sci, Nanjing, Jiangsu, Peoples R China

2.Guangxi Acad Agr Sci, Maize Res Inst, Sweet Potato Lab, Nanning, Guangxi, Peoples R China

3.CAAS, Sweet Potato Res Inst, Xuzhou Acad Agr Sci, Xuzhou, Jiangsu, Peoples R China

关键词： Cylas formicarius; odorant-binding proteins; fluorescence competitive binding; RNA interference; behavior response

期刊名称：PEST MANAGEMENT SCIENCE （ 影响因子：4.845； 五年影响因子：4.674 ）

ISSN： 1526-498X

年卷期：

页码：

收录情况： SCI

摘要： Background The sweet potato weevil,Cylas formicarius, is the most serious pest of sweet potato worldwide. The molecular mechanism of sex pheromone recognition inC. formicariushas not been reported. Odorant-binding proteins (OBPs) play a critical role in selectively binding and transporting pheromones or other odors to the surface of olfactory receptor neurons through the aqueous sensillar lymph, therefore the function of sweet potato OBPs is worth studying. Results Herein, theCforOBP1-3genes encoding three classical OBPs were cloned inC. formicariusby reverse transcription-polymerase chain reaction. Phylogenetic analysis showed thatCforOBP1-3were homologous genes, but the relationship betweenCforOBP2andCforOBP3was closest among the three genes. In addition, real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) assays demonstrated that the expression ofCforOBP1was higher in the antennae and legs of female and male insects, whileCforOBP2andCforOBP3were mainly expressed in the antennae of male insects. The fluorescent competitive binding assay results indicated that CforOBP1-3 had strong binding affinities to sex pheromones and other tested ligands. Finally, the mRNA expression ofCforOBP1-3was successfully inhibited by RNA interference, andin vivobehavioral experiments showed that CforOBP1-3-deficientC. formicariuswas partly anosmic and lost some of its ability to locate sex pheromones and host plant volatiles. Conclusion These results suggested that CforOBP1 was shown to be involved in the process of weevils feeding and finding sweet potato, and CforOBP2-3 were mainly involved in the mating behavior of adult male weevils.