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Expression of kenaf mitochondrial chimeric genes HM184 causes male sterility in transgenic tobacco plants

文献类型： 外文期刊

作者： Zhao, Yanhong 2 ; Liao, Xiaofang 1 ; Huang, Zhipeng 1 ; Chen, Peng 1 ; Zhou, Bujin 1 ; Liu, Dongmei 1 ; Kong, Xiangjun 1 ;

作者机构： 1.Guangxi Univ, Coll Agr, Nanning 530005, Peoples R China

2.Guangxi Acad Agr Sci, Cash Crop Inst, Nanning, Peoples R China

关键词： Cytoplasmic male sterility;kenaf (Hibiscus cannabinus L.);mitochondrial chimeric genes HM184;transgenic plants

期刊名称：MITOCHONDRIAL DNA （ 影响因子：0.925； 五年影响因子：0.994 ）

ISSN： 1940-1736

年卷期： 2015 年 26 卷 4 期

页码：

收录情况： SCI

摘要： Chimeric genes resulting from the rearrangement of a mitochondrial genome were generally thought to be a causal factor in the occurrence of cytoplasmic male sterility (CMS). In the study, earlier we reported that identifying a 47 bp deletion at 3'-flanking of atp9 that was linked to male sterile cytoplasm in kenaf. The truncated fragment was fused with atp9, a mitochondrial transit signal (MTS) and/or GFP, comprised two chimeric genes MTS-HM184-GFP and MTS-HM184. The plant expression vector pBI121 containing chimeric genes were then introduced to tobacco plants by Agrobacterium-mediated T-DNA transformation. The result showed that certain transgenic plants were male sterility or semi-sterility, while some were not. The expression analysis further demonstrated that higher level of expression were showed in the sterility plants, while no expression or less expression in fertility plants, the levels of expression of semi-sterility were in between. And the sterile plant (containing MTS-HM184-GFP) had abnormal anther produced malformed/shriveled pollen grains stained negative that failed to germinate (0%), the corresponding fruits was shrunken, the semi-sterile plants having normal anther shape produced about 10-50% normal pollen grains, the corresponding fruits were not full, and the germination rate was 58%. Meanwhile these transgenic plants which altered on fertility were further analyzed in phenotype. As a result, the metamorphosis leaves were observed in the seedling stage, the plant height of transgenic plants was shorter than wild type. The growth duration of transgenic tobacco was delayed 30-45 days compared to the wild type. The copy numbers of target genes of transgenic tobacco were analyzed using the real-time quantitative method. The results showed that these transgenic plants targeting-expression in mitochondrial containing MTS-HM184-GFP had 1 copy and 2 copies, the other two plants containing MTS-HM184 both had 3 copies, but 0 copy in wild type. In summary, the two manual chimeric genes might be related to male sterility in kenaf.

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[1]A comparative study of the atp9 gene between a cytoplasmic male sterile line and its maintainer line and further development of a molecular marker specific for male sterile cytoplasm in kenaf (Hibiscus cannabinus L.). Chen, Peng,Liao, Xiaofang,Zhou, Bujin,Liao, Jian,Huang, Zhipeng,Kong, Xiangjun,Zhou, Ruiyang,Zhao, Yanhong.

[2]Mutation in the coding sequence of atp6 are associated with male sterile cytoplasm in kenaf (Hibiscus cannabinus L.). Liao, Xiaofang,Zhou, Bujin,Zhou, Yaoyao,Zhou, Ruiyang,Zhao, Hongtao,Zhou, Yaoyao.

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