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Enterobacter sacchari sp nov., a nitrogen-fixing bacterium associated with sugar cane (Saccharum officinarum L.)

文献类型： 外文期刊

作者： Zhu, Bo 1 ; Zhou, Qing 1 ; Lin, Li 2 ; Hu, Chunjin 4 ; Shen, Ping 5 ; Yang, Litao 3 ; An, Qianli 1 ; Xie, Guanlin 1 ; Li, Ya 1 ;

作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Rice Biol, Hangzhou 310029, Zhejiang, Peoples R China

2.Guangxi Acad Agr Sci, Guangxi Key Lab Sugarcane Genet Improvement, Nanning 530007, Peoples R China

3.Guangxi Univ, State Key Lab Conservat & Utilizat Subtrop Agrobi, Nanning 530005, Peoples R China

4.Guangxi Acad Agr Sci, Inst Microbiol, Nanning 530007, Peoples R China

5.Zhejiang Univ, Affiliated Hosp 1, Coll Med, State Key L

期刊名称：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY （ 影响因子：2.747； 五年影响因子：2.889 ）

ISSN： 1466-5026

年卷期： 2013 年 63 卷

页码：

收录情况： SCI

摘要： Five nitrogen-fixing bacterial strains (SP1(T), NN143, NN144, NN208 and HX148) were isolated from stem, root or rhizosphere soil of sugar cane (Saccharum officinarum L.) plants. Cells were Gram-negative, motile, rods with peritrichous flagella. DNA G+C content was 55.0 +/- 0.5 mol%. Sequence determinations and phylogenetic analysis of 16S rRNA gene and rpoB indicated that the strains were affiliated with the genus Enterobacter and most closely related to E. radicincitans DSM 16656(T) and E. oryzae LMG 24251(T). Fluorimetric determination of thermal denaturation temperatures after DNA-DNA hybridization, enterobacterial repetitive intergenic consensus PCR and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry differentiated the whole-genome, genotype and protein profiles from those of E. radicincitans and E. oryzae. The strains' cell fatty acid composition differentiated them from E. radicincitans and E. oryzae by containing a higher level of summed feature 2 (C-16:1 omega 7c and/or C-16:1 omega 6C) and a lower level of C-17:0 cyclo. Their physiological and biochemical profiles differentiated them from E. radicincitans by being positive for methyl red test, ornithine decarboxylase and utilization of putrescine, D-arabitol, L-fucose and methyl alpha-D-glucoside and being negative for arginine dihydrolase, and differentiated them from E. oryzae by being positive for aesculin hydrolysis and utilization of putrescine, D-arabitol and L-rhamnose and being negative for arginine dihydrolase, lysine decarboxylase and utilization of mucate. The five strains therefore represent a novel species, for which the name Enterobacter sacchari sp. nov. is proposed, with the type strain SP1(T) (=CGMCC 1.12102(T)= LMG 26783(T)).

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