文献类型： 外文期刊

作者： Ye Yun-feng ² ; Li Qi-qin ¹ ; Fu Gang ³ ; Yuan Gao-qing ¹ ; Miao Jian-hua ² ; Lin Wei ¹ ;

作者机构： 1.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China

2.Guangxi Bot Garden Med Plant, Nanning 530023, Peoples R China

3.Guangxi Acad Agr Sci, Microbiol Res Inst, Nanning 530007, Peoples R China

关键词： identification;Bipolaris maydis;biocontrol

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2012 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Bacillus subtilis B47 is an endophytic bacterium of tomato, and produces substance that strongly inhibits the growth of Bipolaris maydis, the pathogen of southern corn leaf blight (SCLB), as well as several other phytopathogenic fungi. The antifungal substance was purified from the broth culture of the bacterium using acid precipitation, methanol extraction, and three-step chromatography. Based on FT-IR spectrometry, amino acid composition, and MALDI-TOF-MS/MS CID analyses, the antifungal substance was identified as iturin A(2), a cyclic lipopeptide antibiotic. To evaluate the efficacy of iturin A(2) for control of SCLB, partially purified iturin A(2) (75%, w/v) was applied under different conditions. At the concentration of 300 mg kg(-1), iturin A(2) showed efficacy ranging from 100 to 53.1% under in vitro, in plot and in field conditions. This efficacy was higher than or similar to that of the fungicide chlorothalonil. When the concentration of iturin A(2) was increased to 500 mg kg(-1), the control efficacy was enhanced to 64.2% in field, which was significantly higher than that of chlorothalonil. These results indicate that iturin A(2) has potential for SCLB control and could be a substitute to synthetic fungicides. To our knowledge, this is the first report on using partially purified iturin A for control of SCLB under field conditions.