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Biochemical properties and potential endogenous substrates of polyphenoloxidase from chufa (Eleocharis tuberosa) corms

文献类型： 外文期刊

作者： Sun, Jian 2 ; You, Yanli 3 ; Garcia-Garcia, Elena 4 ; Long, Xing 1 ; Wang, Jubing 6 ;

作者机构： 1.Guangxi Acad Agr Sci, Inst Hort Res, Nanning 530007, Guangxi Prov, Peoples R China

2.Guangxi Crop Genet Improvement & Biotechnol Lab, Nanning 530007, Peoples R China

3.Yantai Univ, Inst Food Sci & Engn, Yantai 264005, Peoples R China

4.Univ Miguel Hernandez, CIBERDEM, Elche 03202, Spain

5.Univ Miguel Hernandez, Inst Bioingn, Elche 03202, Spain

6.Guangxi Tech Instruct Gen Off Fruit Prod, Nanning 530022, Peoples R China

关键词： Chufa;Corms;Polyphenoloxidase;Potential endogenous substrates;Polyphenols

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN： 0308-8146

年卷期： 2010 年 118 卷 3 期

页码：

收录情况： SCI

摘要： Polyphenoloxidase (PPO) was partially purified from chufa corms through ammonium sulfate precipitation and dialysis. Biochemical properties of chufa PPO were analysed using exogenous substrate catechol. Optimal pH and temperature for PPO activity were 5 and 45 degrees C. Ethylenediaminetetraacetic acid disodium salt and L-cysteine could not inhibit the PPO activity. However, sodium thiosulfate pentahydrate exhibited the strongest inhibiting effect, followed by ascorbic acid and anhydrous sodium sulfite. Except for K+, other metal ions such as Zn2+, Cu2+, Fe3+, Ca2+, Fe2+ and Na+ accelerated the enzymatic reaction between catechol and PPO. Kinetic analysis showed that the apparent K-m and V-max values were around 10.77 mM and 82 units/ml min. In addition, (-)-gallocatechin gallate, (-)-epicatechin gallate and (+)-catechin gallate isolated and identified from chufa corms were supposed to be the potential endogenous PPO substrates due to their ortho-diphenolic or pyrogallolic structures. These polyphenols might be catalysed by PPO, resulting in the browning of chufa corms after fresh-cut processing. (C) 2009 Elsevier Ltd. All rights reserved.

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