文献类型: 外文期刊
作者: Huang, JF 1 ; Zhou, XP 1 ; Cai, JH 1 ; Li, GX 1 ;
作者机构: 1.Zhejiang Univ, Inst Biotechnol, Hangzhou 310029, Peoples R China; Shihezi Univ, Key Lab Oasis Ecol Agr Xinjiang Bingtuan, Shihexi, Peoples R China; Guangxi Acad Agr Sci, Inst Plant Protect, Nanning, Peoples R China
关键词: begomovirus;cloning;DNA-A;PCR;phylogenetic analysis;sequencing;Senecio yellow mosaic virus;Senecio scandens
期刊名称:ACTA VIROLOGICA ( 影响因子:1.162; 五年影响因子:1.255 )
ISSN: 0001-723X
年卷期: 2005 年 49 卷 3 期
页码:
收录情况: SCI
摘要: Three begomovirus isolates, G46, G83 and G84 from Senecio scandens showing yellow mosaic symptoms were collected from Guangxi Province, P.R. China. The isolates were detected by PCR using universal primers for begomoviruses. Comparison of partial DNA-A sequences (similar to 500 bp) of the isolates revealed their 98.7-99.1% identity. The isolate G46, chosen for complete DNA-A sequencing, consisted of 2746 nt and had a typical genomic organization of begomoviruses. The G46 DNA-A had the highest sequence identity (72.4%) with that of Ageratum leaf curl virus among begomoviruses. The molecular data suggest that the isolate G46 is a new begomovirus (species), for which the name Senecio yellow mosaic virus (Senecio yellow mosaic virus) is proposed.
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