文献类型： 外文期刊

作者： Chen, Zhao Jie ¹ ; Shi, Xu Zhen ¹ ; He, Zhi Hai ¹ ; Qu, Ya Nan ¹ ; Ai, Gan ³ ; Wang, Yan Hui ⁴ ; Wang, Yi Zhuo ¹ ; Yang, Hong ² ;

作者机构： 1.Guangxi Univ, Coll Agr, Guangxi Key Lab Agr Environm & Agr Prod Safety, Da Xue East Rd 100, Nanning 530004, Guangxi, Peoples R China

2.Nanjing Agr Univ, Coll Sci, Jiangsu Key Lab Pesticide Sci, Nanjing 210095, Peoples R China

3.Nanjing Agr Univ, Coll Plant Protect, Key Lab Plant Immun, Nanjing 210095, Peoples R China

4.Guangxi Acad Agr Sci, Plant Protect Res Inst, Guangxi Key Lab Biol Crop Dis & Insect Pests, Nanning 530007, Peoples R China

关键词： AP2 transcription factor; Mesotrione; Rice; Bioinformatics analysis; Metabolism

期刊名称：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE （ 影响因子：6.6； 五年影响因子：6.6 ）

ISSN：

年卷期： 2024 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Background The APETALA2 (AP2) transcription factor (TF) superfamily, one of the largest gene families in plants, plays an essential role in regulating plant growth and their stress responses. However, the role of AP2 in rice under pesticide stress remains unclear. To investigate the characteristics and functions of the rice AP2 gene family under pesticide stress, the expression of 105 AP2-coding genes and 26 AP2 differentially expressed genes (DEGs) were identified in mesotrione (MTR)-treated rice transcriptome datasets. Results Three subfamilies of the AP2 gene family (AP2/ERF, RAV, and soloists) were identified using sequence alignment and phylogenetic analysis. Chromosome location analysis revealed that the 26 rice AP2 DEGs were unevenly distributed on 10 of the 12 rice chromosomes, and segmental duplication contributed to the expansion of Oryza sativa AP2 (OsAP2) gene family. Collinearity analyses demonstrated that rice AP2 genes displayed 16 orthologous gene pairs, and 12 and 26 orthologous gene pairs were shared of Arabidopsis and soybean, respectively. In addition, rice AP2 genes featured various gene structures, cis-elements, motif compositions, and conserved domains that allowed them to encode genes that elicit biotic and abiotic stress responses. An analysis of docking between MTR and six AP2 DEGs revealed amino acid residues involved in MTR binding. Quantitative reverse transcription-polymerase chain reaction verified that several AP2 genes were preferentially expressed during MTR-induced stress. The roles of OsAP2 proteins in MTR metabolism were further supported by protein-protein interaction network analysis, which illustrated how these proteins interact with target proteins. Conclusion The initial findings of this study define the features of the OsAP2 superfamily and offer important tools for functional analyses of OsAP2 genes implicated in the metabolism of MTR.