文献类型： 会议论文

第一作者： He XH

作者： He XH ¹ ; Guo YZ ¹ ; Jiang YQ ¹ ; She JC ¹ ; Li YR ² ;

作者机构： 1.College of Agriculture, Guangxi University, Nanning 530004, P. R. China

2.Guangxi Crop Genetic Improvement and Biotechnology Lab, Guangxi Academy of Agricultural Sciences, Nanning 530007, P.R. China

关键词： Citrus unshiu Marcovitch;Citrus reticulata Blanco;Citrus grandis ( L. )Osbeck;Fortunella crassifolia Swingle;Clausena lansium ( Lour. ) Skeels

会议名称： International citrus congress

主办单位：

页码： 418-421

摘要： LEAFY (LFY) gene not only controls flowering time and floral transition, but also plays an important role in inflorescence and floral organ development. It was situated at the central site in gene network of flowering regulation. In order to probe further the molecular mechanism among Citrus and its relative plants, complete LFY homologous genes from Satsuma (Citrus unshiu Marcovitch) , Ponkan (C. reticulata Blanco) , Shatian pomelo [ C. grandis (L.)Osbeck ] , Kumquat (Fortunella crassifolia Swingle) and Wompee [Clausena lansium (Lour.)Skeels] were isolated and cloned by polymerase chain reaction (PCR). The results showed that the nucleotide size of complete LFY homologous genes from Satsuma, Ponkan, Shatian pomelo, Kumquat and Wompee was 2 090bp, 2 086bp, 2 092bp, 2 081bp and 2 089bp respectively, and they encoded 398, 398, 398, 398 and 397 amino acids respectively. These complete LFY homologous genes were all composed of 3 exons and 2 introns. The homologies of nucleotide and amino acid sequences among the complete LFY homologous genes were 92% -99% and 95% - 100% respectively. The result of phylogenetic tree based on amino acid sequence homology among LFY homologous is similar to the result of plant taxonomy.

分类号： S666`S666